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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab108080-10μl
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10μl |
2
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$66.90
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Ab108080-50μl
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50μl |
1
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$199.90
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Ab108080-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab108080-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,809.90
|
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Recombinant; Rabbit anti Human Histone H4 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant Histone H4 Antibody - Primary antibody, specific to Histone H4, Rabbit IgG |
|---|---|
| Synonyms | dJ160A22.1 antibody | dJ160A22.2 antibody | dJ221C16.1 antibody | dJ221C16.9 antibody | FO108 antibody | H4 antibody | H4.k antibody | H4/a antibody | H4/b antibody | H4/c antibody | H4/d antibody | H4/e antibody | H4/g antibody | H4/h antibody | H4/I ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | Histone H4 |
| Immunogen | A synthetic peptide derived from human Histone H4 (AA 60-76). |
| Positive Control | WB: HeLa, HEK293, A431 and Hep G2 cell lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Histone H4 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant Histone H4 Antibody (Ab108080) - Western Blot
All lanes: Recombinant Histone H4 Antibody (Ab108080) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Exposure time: 10.0 s
Recombinant Histone H4 Antibody (Ab108080) - IF/ICC
Immunocytochemistry analysis of Histone H4 (green) in HeLa using Recombinant Histone H4 Antibody (Ab108080), and cells were counterstained with DAPI (blue).
Recombinant Histone H4 Antibody (Ab108080) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant Histone H4 Antibody (Ab108080). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
| WB |
1/500-1/1000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Feb 23, 2024 | Ab108080 | |
| Certificate of Analysis | Feb 23, 2024 | Ab108080 |
Starting at $99.90