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Goat Anti-Rabbit IgG H&L (Cy5) - Secondary antibody, Goat IgG

    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: Cy5
In stock
Item Number
Ab176445
Grouped product items
SKU Size
Availability
Price Qty
Ab176445-100μg
100μg
≥10
$29.90
Ab176445-1mg
1mg
4
$169.90

Secondary Antibody; Goat Anti-Rabbit IgG H&L (Cy5); IF, Flow

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (Cy5) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Rabbit IgG H&L Antibody (Cy5) | Anti-Rabbit IgG H&L Antibody (Cy5) | Rabbit IgG H&L Antibody (Cy5) | Cy5-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (Cy5) | Anti-Rabbit IgG Antibody (Cy5) | Rabbit IgG Antibody (Cy5) | G
Specifications & Purity ExactAb™, Validated, Azide Free, Ex:647nm, Em:665nm
Host species Goat
Immunogen Rabbit IgG
Positive Control 1.0 mg/mL
Conjugation Cy5
Excitation(nm) Red Laser(633nm)
Emission(nm) 665nm
Ex/Em(nm) Ex:647nm;Em:665nm
Grade Azide Free, Em:665nm, Ex:647nm, ExactAb™, Validated
Product Description

Application
IF: 1:1000
Flow: 1:500-1:2500

Product Properties

Isotype Goat IgG
Purification Method Antigen affinity purified
Shape Liquid
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (Cy5) (Ab176445) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Cy5) (Ab176445) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1:1000

Flow Cytometry

1:500-1:2500

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101818 Certificate of Analysis Nov 24, 2023 Ab176445
ZJ23F1101815 Certificate of Analysis Nov 24, 2023 Ab176445

Alternative Products

Documents & Articles

Caspase Immunofluorescence Staining Protocol
General Protocol for Western Blotting
Western Blotting with Fluorescently Labeled Antibodies
Immunofluorescence Staining Protocol (Methanol permeabilization)
Guidelines of Secondary Antibody for Western Blotting
Flow Cytometry Using Methanol Permeabilization
CLARITY Tissue Clearing Staining Technology
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry

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