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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab176445-100μg
|
100μg |
≥10
|
$29.90
|
|
|
Ab176445-1mg
|
1mg |
4
|
$169.90
|
|
Secondary Antibody; Goat Anti-Rabbit IgG H&L (Cy5); IF, Flow
| Product Name | Goat Anti-Rabbit IgG H&L (Cy5) - Secondary antibody, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Rabbit IgG H&L Antibody (Cy5) | Anti-Rabbit IgG H&L Antibody (Cy5) | Rabbit IgG H&L Antibody (Cy5) | Cy5-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (Cy5) | Anti-Rabbit IgG Antibody (Cy5) | Rabbit IgG Antibody (Cy5) | G |
| Specifications & Purity | ExactAb™, Validated, Azide Free, Ex:647nm, Em:665nm |
| Host species | Goat |
| Immunogen | Rabbit IgG |
| Positive Control | 1.0 mg/mL |
| Conjugation | Cy5 |
| Excitation(nm) | Red Laser(633nm) |
| Emission(nm) | 665nm |
| Ex/Em(nm) | Ex:647nm;Em:665nm |
| Grade | Azide Free, Em:665nm, Ex:647nm, ExactAb™, Validated |
| Product Description |
Application |
| Isotype | Goat IgG |
|---|---|
| Purification Method | Antigen affinity purified |
| Shape | Liquid |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
Goat Anti-Rabbit IgG H&L (Cy5) (Ab176445) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Cy5) (Ab176445) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 1:1000 |
| Flow Cytometry | 1:500-1:2500 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Nov 24, 2023 | Ab176445 | |
| Certificate of Analysis | Nov 24, 2023 | Ab176445 |
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