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Goat Anti-Rabbit IgG H&L (TRITC) - Secondary antibody, Goat IgG

    Application:
  • IF/ICC
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: TRITC
In stock
Item Number
Ab176448
Grouped product items
SKU Size
Availability
Price Qty
Ab176448-100μg
100μg
≥10
$19.90
Ab176448-1mg
1mg
1
$89.90

Secondary Antibody; Goat Anti-Rabbit IgG H&L (TRITC); IF

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (TRITC) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Rabbit IgG H&L Antibody (TRITC) | Anti-Rabbit IgG H&L Antibody (TRITC) | Rabbit IgG H&L Antibody (TRITC) | TRITC -conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (TRITC) | Anti-Rabbit IgG Antibody (TRITC) | Rabbit IgG Antib
Specifications & Purity ExactAb™, Validated, Azide Free, Ex:557nm, Em:576nm, 1.0 mg/mL
Host species Goat
Immunogen Rabbit IgG
Conjugation TRITC
Excitation(nm) Green Laser(532nm)
Emission(nm) 576nm
Ex/Em(nm) Ex:557nm;Em:576nm
Grade Azide Free, Em:576nm, Ex:557nm, ExactAb™, Validated
Product Description

Application
IF: 1:1000

Product Properties

Isotype Goat IgG
Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1:1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101796 Certificate of Analysis Nov 24, 2023 Ab176448
ZJ23F1101795 Certificate of Analysis Nov 24, 2023 Ab176448

Alternative Products

Documents & Articles

RNA dot blot protocol
Caspase Immunofluorescence Staining Protocol
General Protocol for Western Blotting
Western Blotting with Fluorescently Labeled Antibodies
Immunofluorescence Staining Protocol (Methanol permeabilization)
Guidelines of Secondary Antibody for Western Blotting
CLARITY Tissue Clearing Staining Technology
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry

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