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Tag IHC
Articles by tag "IHC"
- IHC principle, operation and precautions Immunohistochemistry (IHC) is a technique that uses the principle of specific binding between antigens and antibodies to locate, characterize and quantify antigens (peptides and proteins) within tissue cells by chemically reacting the marker antibody's color developer (fluorescein, enzyme, metal ...
- What does IHC mean on the data sheet? Is it frozen section? Is it different from IHC-F? What does IHC mean on the data sheet? Is it frozen section? Is it different from IHC-F?
- How many slices can I do with 20μI when I have IHC lab-applicable antibodies? How many slices can I do with 20μI when I have IHC lab-applicable antibodies?
- IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate? IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?
- Automated Immunohistochemical Staining Protocol for Anti-PD-L1 Automated Immunohistochemical Staining Protocol for Anti-PD-L1
- Immunohistochemical staining protocol for frozen samples (IHC-Fr) Immunohistochemical staining protocol for frozen samples (IHC-Fr)
- Immunohistochemistry (IHC) staining antigen retrieval protocol Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...
- Protocol and tips for whole mount immunohistochemical staining Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning.
- Antibody applications and techniques Antibodies are powerful research tools used in a variety of laboratory techniques. Here, we will provide a brief overview of the most popular laboratory techniques, focusing on how they use antibodies.