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Protocols
- Immunofluorescence Protocol Immunofluorescence Protocol
- Western Blot Protocol Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.
- Flow Cytometry Experimental Steps for Methanol Permeabilization & Triton X-100 Permeabilization Experimental Steps for Methanol Permeabilization & Triton X-100 Permeabilization
- Deproteinization Using Perchloric Acid Precipitation Protocol In biological sample analysis, the presence of proteins often interferes with small molecule detection, and some enzyme activities may affect the analysis results. Perchloric acid (PCA) precipitation has become a widely used method due to its ability to efficiently remove proteins and its ...
- Bielschowsky Silver Staining Technique Bielschowsky silver staining is a classic silver staining technique, which is mainly used in the pathological study of nervous system tissues, especially in showing structures such as neuronal axons, neurofibrillary tangles and senile plaques.
- Sodium Azide Removal Protocol Sodium azide is a commonly used preservative to prevent bacterial and fungal contamination in antibody solutions. However, sodium azide is cytotoxic, interferes with antibody coupling reactions, and inhibits the activity of enzymes such as horseradish peroxidase (HRP).
- CLARITY Tissue Clearing Staining Technology CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/Insitu hybridization-compatible Tissue hYdrogel) is a revolutionary tissue clearing and staining technology that enables three-dimensional imaging and analysis of intact biological tissues by combining hydrogel ...
- Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry The detection of cell surface proteins mainly targets antigens on the cell membrane. Antibodies can directly bind to antigens on the cell surface, so fixation and permeabilization treatment are usually not required. The experimental operation is relatively simple, and the main focus is on the ...
- Bioorthogonal Chemistry Bioorthogonal chemistry is a set of reactions that can take place in biological environments without affecting biomolecules or interfering with biochemical processes. For this purpose, the reaction must meet the following requirements: fast, efficient, and specific
- Azide-Alkyne Click Chemistry Classic Click Chemistry uses copper, Cu(I), to catalyze the 1,3-dipolar cycloaddition of an alkyne with an azide to form a 1,2,3-triazole.1,2 Sources of Cu(I) include copper(I) iodide, copper(I) bromide, copper turnings, or copper (II) sulfate (CuSO4) and a reducing agent.1 However, the ...