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Clonage & expression des protéines
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T4 DNA PolymeraseSpecies: Escherichia phage T4 Accession #: P04415Système d'expression: E. coliBioactivité: Catalytic incorporation of 10nmol deoxyribonucleotides (dNTPs) into polynucleotides at 37 ℃ for 30 minutes. The required enzyme quantity is defined as one active unit.Synonymes: T4DNAPolymerase | Eta2 | DNA-directed DNA polymerase
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5' DNA/RNA AdenylaseSpecies: Adenosylase gene of thermophilic bacteria Accession #: 8UCH_A,A0A075LQ94Système d'expression: E. coliBioactivité: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.Synonymes: Single-Strand Nucleic Acid 5' Adenylase
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Système d'expression: E. coliBioactivité: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
