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Histone H4 Mouse mAb - Primary antibody, specific to Histone H4, IgG2b

COA COA
    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: CD01/2H7
  • Conjugation: Unconjugated
In stock
Item Number
Ab156574
Grouped product items
SKU Size
Availability
 Price Qty
Ab156574-10μl
10μl
≥10
$99.90
Ab156574-50μl
50μl
≥10
$199.90
Ab156574-100μl
100μl
3
$299.90
Ab156574-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CD01/2H7); Mouse anti Vinculin Antibody; WB, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: HIST1H4A
Accession#:P62805 (5) Gene ID:8359 (4) H4C1 (5) Histone H4 (5)

Basic Description

Product Name Histone H4 Mouse mAb - Primary antibody, specific to Histone H4, IgG2b
Synonyms dJ160A22.1 antibody | dJ160A22.2 antibody | dJ221C16.1 antibody | dJ221C16.9 antibody | FO108 antibody | H4 antibody | H4.k antibody | H4/a antibody | H4/b antibody | H4/c antibody | H4/d antibody | H4/e antibody | H4/g antibody | H4/h antibody | H4/I ant
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Host species Mouse
Specificity Histone H4
Immunogen Recombinant full length protein corresponding to Human H4C1
Positive Control WB: Hela, A431, MCF-7, NIH/3T3, C6, Jurkat, K562, A549, LNCaP whole cell lysates ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/1,000-1/200,000 ; Predicted molecular weight: 11.4 kDa
ICC/IF: 1.0 μg/mL

Product Properties

Isotype IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HIST1H4A Tchem Histone H4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Histone H4 Mouse mAb (Ab156574) - Western Blot
All lanes: Histone H4 Antibody (Ab156574) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 11.4 kDa
Observed band size: 13.6 kDa
Exposure time: 2.4 s 

Histone H4 Mouse mAb (Ab156574) - Western Blot
All lanes: Histone H4 Antibody (Ab156574) at 1/10000 dilution
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 11.4 kDa
Observed band size: 13.5 kDa
Exposure time: 60.0 s 

Histone H4 Mouse mAb (Ab156574) - ICC/IF
IF analysis of Histone H4 (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Histone H4 Mouse mAb (Ab156574) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
WB

1/1,000-1/200,000 ; Predicted molecular weight: 11.4 kDa
IF/ICC

1.0 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0102555 Certificate of Analysis Jan 23, 2024 Ab156574
ZJ24F0102556 Certificate of Analysis Jan 23, 2024 Ab156574
ZJ24F0102557 Certificate of Analysis Jan 23, 2024 Ab156574

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