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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab176442-100μg
|
100μg |
≥10
|
$19.90
|
|
|
Ab176442-1mg
|
1mg |
2
|
$109.90
|
|
Secondary Antibody; Goat Anti-Rabbit IgG H&L (Biotin); WB, ELISA, IF
| Product Name | Goat Anti-Rabbit IgG H&L (Biotin) - Secondary antibody, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Rabbit IgG H&L Antibody (Biotin) | Anti-Rabbit IgG H&L Antibody (Biotin) | Rabbit IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (Biotin) | Anti-Rabbit IgG Antibody (Biotin) | Rabbit IgG |
| Specifications & Purity | ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL |
| Host species | Goat |
| Immunogen | Rabbit IgG |
| Conjugation | Biotin |
| Grade | Azide Free, ExactAb™, High Performance, Validated |
| Product Description |
Application ELISA: 1:10,000-1:200,000 |
| Isotype | Goat IgG |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Antigen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing |
| Shipped In | Wet ice |
| Stability And Storage | Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) at 1/40000 dilution
SA-HRP:1:20000
Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 7.0 s
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - ELISA
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
| Application | Dilution info |
|---|---|
| WB | 1/10000-1/200000 |
| ELISA | 1:10000-1:200000 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Dec 26, 2024 | Ab176442 | |
| Certificate of Analysis | Nov 24, 2023 | Ab176442 | |
| Certificate of Analysis | Nov 24, 2023 | Ab176442 |
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