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Goat Anti-Rabbit IgG H&L (AF647) - Secondary antibody, Goat IgG

COA COA
    Application:
  • IF/ICC
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: AF647
In stock
Item Number
Ab181958
Grouped product items
SKU Size
Availability
 Price Qty
Ab181958-10μg
10μg
≥10
$19.90
Ab181958-100μg
100μg
≥10
$39.90
Ab181958-1mg
1mg
3
$99.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Goat Anti-Rabbit IgG H&L Antibody (AF647); IF

View related series
AF647 (119) CLARITY tissue clearing technology (64) Goat Anti-Rabbit IgG (10) Goat Anti-Rabbit IgG H&L (AF647) (2)

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (AF647) - Secondary antibody, Goat IgG
Synonyms BCM1 surface antigen | BCM1 | BCM1Leukocyte antigen MEM-102 | BLAST | BLAST1 | BLAST1TCT.1 | B-lymphocyte activation marker BLAST-1 | CD48 antigen (B-cell membrane protein) | CD48 antigen | CD48 molecule | CD48 | hCD48 | mCD48 | MEM-102 | SLAMF2 | Antigen
Specifications & Purity ExactAb™, Validated, Ex:650nm, Em:668nm, 1.0 mg/mL
Host species Goat
Immunogen Rabbit IgG
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Application
IF: 1:100-1:1000.

Product Properties

Isotype Goat IgG
Purification Method antigen affinity chromatography
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) - IF
IF analysis of MMP9 (red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark. beta Tubulin Mouse mAb (Ab179922) (green) was stained with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at 1/1000 dilution. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) - IF
IF analysis of MMP9 (red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) - IF
IF analysis of Alpha Tubulin (red) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant Alpha Tubulin Antibody (Ab088473) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0707760 Certificate of Analysis Jul 03, 2024 Ab181958
ZJ24F0707759 Certificate of Analysis Jul 03, 2024 Ab181958
ZJ24F0707761 Certificate of Analysis Jul 03, 2024 Ab181958
ZJ24R0600730 Certificate of Analysis Jun 21, 2024 Ab181958

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