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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab181956-10μg
|
10μg |
5
|
$15.90
|
|
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Ab181956-100μg
|
100μg |
5
|
$59.90
|
|
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Ab181956-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$359.90
|
|
pAb; Goat Anti-Rabbit IgG H&L Antibody (AF555); IF
| Product Name | Goat Anti-Rabbit IgG H&L (AF555) - Secondary antibody, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Rabbit IgG H&L Antibody | Goat Anti Rabbit IgG H&L Antibody | Anti-Rabbit IgG H&L Antibody | Anti Rabbit IgG H&L Antibody | Goat Anti-Rabbit IgG Antibody | Goat Anti Rabbit IgG Antibody | Anti- Rabbit IgG Antibody | Anti Rabbit IgG Antibody | AF |
| Specifications & Purity | ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system. |
| Host species | Goat |
| Immunogen | Rabbit IgG |
| Conjugation | AF555 |
| Excitation(nm) | Green Laser(532nm) |
| Emission(nm) | 568nm |
| Ex/Em(nm) | Ex:553nm;Em:568nm |
| Grade | Em:568nm, Ex:553nm, ExactAb™, Validated |
| Product Description |
Goat Anti-Rabbit IgG H&L Antibody (AF555), Secondary antibody, could be used for IF
|
| Isotype | Goat IgG |
|---|---|
| Purification Method | antigen affinity chromatography |
| Concentration | 1.0 mg/mL |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C. Avoid freeze/thaw cycle. Protect from light. |
Goat Anti-Rabbit IgG H&L (AF555) (Ab181956) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 0.5 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF555) (Ab181956) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/100-1/1000
|
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