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Goat Anti-Rabbit IgG H&L (AF555) - Secondary antibody, Goat IgG

    Application:
  • IF/ICC
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: AF555
In stock
Item Number
Ab181956
Grouped product items
SKU Size
Availability
Price Qty
Ab181956-10μg
10μg
5
$15.90
Ab181956-100μg
100μg
5
$59.90
Ab181956-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$359.90

pAb; Goat Anti-Rabbit IgG H&L Antibody (AF555); IF

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (AF555) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Rabbit IgG H&L Antibody | Goat Anti Rabbit IgG H&L Antibody | Anti-Rabbit IgG H&L Antibody | Anti Rabbit IgG H&L Antibody | Goat Anti-Rabbit IgG Antibody | Goat Anti Rabbit IgG Antibody | Anti- Rabbit IgG Antibody | Anti Rabbit IgG Antibody | AF
Specifications & Purity ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL
Biochemical and Physiological Mechanisms IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Host species Goat
Immunogen Rabbit IgG
Conjugation AF555
Excitation(nm) Green Laser(532nm)
Emission(nm) 568nm
Ex/Em(nm) Ex:553nm;Em:568nm
Grade Em:568nm, Ex:553nm, ExactAb™, Validated
Product Description

Goat Anti-Rabbit IgG H&L Antibody (AF555), Secondary antibody, could be used for IF

Product Properties

Isotype Goat IgG
Purification Method antigen affinity chromatography
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (AF555) (Ab181956) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 0.5 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF555) (Ab181956) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/100-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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