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Goat Anti-Rabbit IgG H&L (HRP) - Secondary antibody, Goat IgG

COA COA
    Application:
  • ELISA
  • WB
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: HRP
In stock
Item Number
Ab176443
Grouped product items
SKU Size
Availability
 Price Qty
Ab176443-100μg
100μg
≥10
$25.90
Ab176443-1mg
1mg
5
$119.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Rabbit IgG H&L (HRP); WB, ELISA, IHC

View related series
Goat Anti-Rabbit (19) HRP (26) mIHC (14) Multiple Fluorescence Immunohistochemistry Experiment (13)

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (HRP) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Rabbit IgG H&L Antibody (HRP) | Anti-Rabbit IgG H&L Antibody (HRP) | Rabbit IgG H&L Antibody (HRP) | Peroxidase-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (HRP) | Anti-Rabbit IgG Antibody (HRP) | Rabbit IgG Antibody (H
Specifications & Purity ExactAb™, High Performance, Validated, Azide Free
Host species Goat
Immunogen Rabbit IgG
Positive Control 1.0 mg/mL
Conjugation HRP
Grade Azide Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1:10,000-1:200,000
ELISA: 1:10,000-1:100,000

Product Properties

Isotype Goat IgG
Purification Method Antigen affinity purified
Shape Liquid
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/40000 dilution

Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 2.0 s

Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) - ELISA
Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) was used as the secondary antibody.

Application

Application Dilution info
WB

1/10000-1/200000
ELISA

1:10000-1:100000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24R0500573 Certificate of Analysis May 19, 2024 Ab176443
ZJ23F1101823 Certificate of Analysis Nov 24, 2023 Ab176443
ZJ23F1101825 Certificate of Analysis Nov 24, 2023 Ab176443

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Documents & Articles

In-cell ELISA protocol
Categories: Protocols
Tags:
Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies
Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol
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Cytokines Growth factors ELISPOT Secreted Proteins HRP Conjugated Secondary Antibodies
Automated Immunohistochemical Staining Protocol for Anti-PD-L1
Categories: Protocols
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PD-L1 IHC Monoclonal antibodies Automated Immunohistochemistry Isotype Control Antibodies
General Protocol for Western Blotting
Categories: Protocols
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Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP
BN-PAGE Protocol
Categories: Protocols
Tags:
Western blotting Natural polypropylene gel electrophoresis Non-denaturating gel electrophoresis BN-PAGE Coomasil brilliant blue Protein complex Horseradish peroxidase Alkaline phosphatase
Histone Western Blot
Categories: Protocols
Tags:
Monoclonal antibodies Western blot Recombinant Antibodies Immunoblotting HRP Horseradish peroxidase Histone
Protocol of Western blot for Phosphorylated Proteins
Categories: Protocols
Tags:
Western blot ECL Phosphorylated proteins Phosphorylated Antibodies Immunoblot HRP-conjugated Antibodies
Guidelines of Secondary Antibody for Western Blotting
Categories: Protocols
Tags:
Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Multiplex Immunohistochemistry Protocol
Categories: Protocols
Tags:
Immunoglobulin Immunohistochemical staining Immunofluorescence TSA HRP labeled secondary antibody Tyrosine amplification signal Multiple immunohistochemistry
Western Blot Protocol
Categories: Protocols
Tags:
WB SDS-PAGE Western blot Cell lysis Protein extraction electrophoresis buffer protease inhibitor phosphatase inhibitor ECL hypersensitive luminescent solution gel preparation chemiluminescent detection

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