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Goat Anti-Rabbit IgG H&L (HRP) - Secondary antibody

COA COA
    Application:
  • ELISA
  • IHC
  • WB
  • Host species: Goat
  • Reactive species: Rabbit
  • Conjugation: HRP
In stock
Item Number
Ab170144
Grouped product items
SKU Size
Availability
 Price Qty
Ab170144-100μl
100μl
2
$19.90
Ab170144-1ml
1ml
1
$99.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Rabbit IgG H&L (HRP); WB, ELISA, IHC

View related series
Goat Anti-Rabbit (19) HRP (26) mIHC (14) Multiple Fluorescence Immunohistochemistry Experiment (13)

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (HRP) - Secondary antibody
Synonyms Goat Anti-Rabbit IgG H&L Antibody (HRP) | Anti-Rabbit IgG H&L Antibody (HRP) | Rabbit IgG H&L Antibody (HRP) | Peroxidase-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (HRP) | Anti-Rabbit IgG Antibody (HRP) | Rabbit IgG Antibody (H
Specifications & Purity ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL
Host species Goat
Immunogen Rabbit IgG
Positive Control ELISA: Rabbit IgG
Conjugation HRP
Grade Azide Free, ExactAb™, High Performance, Pre-adsorbed, Validated
Product Description

HRP conjugated anti-rabbit secondary antibody is commonly used for immunohistochemistry, Western blotting, and ELISA.
Application
WB: 1:10000-1:200000
ELISA: 1:5000-1:100000
IHC: Assay-dependent

Product Properties

Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) - Western Blot
All lanes: Recombinant gamma Tubulin Antibody (Ab104713) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 48 kDa
Exposure time: 1.2 s

Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) - ELISA
Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) was used as the secondary antibody.

Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) - ELISA
Cross-reactivity of Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution was used as the primary antibody.

Application

Application Dilution info
WB

1/10000-1/200000
ELISA

1/5000-1/100000
IHC

Assay-dependent

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901192 Certificate of Analysis Jun 25, 2024 Ab170144
ZJ23F0901193 Certificate of Analysis Jun 25, 2024 Ab170144

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Guidelines of Secondary Antibody for Western Blotting
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