Product Name	Goat Anti-Rabbit IgG H&L (AF405) - Secondary antibody, Goat IgG
Synonyms	Goat Anti-Rabbit IgG H&L Antibody \| Goat Anti Rabbit IgG H&L Antibody \| Anti-Rabbit IgG H&L Antibody \| Anti Rabbit IgG H&L Antibody \| Goat Anti-Rabbit IgG Antibody \| Goat Anti Rabbit IgG Antibody \| Anti- Rabbit IgG Antibody \| Anti Rabbit IgG Antibody \| AF
Specifications & Purity	ExactAb™, Validated, Ex:401nm, Em:422nm, 1.0 mg/mL
Biochemical and Physiological Mechanisms	IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Host species	Goat
Immunogen	Rabbit IgG
Conjugation	AF405
Excitation(nm)	Violet Laser(405nm)
Emission(nm)	422nm
Ex/Em(nm)	Ex:401nm;Em:422nm
Grade	Em:422nm, Ex:401nm, ExactAb™, Validated
Product Description	Goat Anti-Rabbit IgG H&L Antibody (AF405), Secondary antibody, could be used for IF

Product Properties

Isotype	Goat IgG
Purification Method	antigen affinity chromatography
Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF: Goat Anti-Rabbit IgG H&L (AF405) (Ab181953)

Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) - IF
IF analysis of MMP9 (blue) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) at a dilution of 1/1000 for 1 hour at room temperature in the dark. beta Tubulin Mouse mAb (Ab179922) (green) was stained with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at 1/1000 dilution. Cells were counterstained with PI (red). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IF: Goat Anti-Rabbit IgG H&L (AF405) (Ab181953)

Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) - IF
IF analysis of MMP9 (blue) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with PI (red). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IF/ICC	1/100-1/1000

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Goat Anti-Rabbit IgG H&L (AF405) - Secondary antibody, Goat IgG

Basic Description