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Tag Western blot
Articles by tag "Western blot"
- Western Blot Protocol Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.
- Guidelines of Secondary Antibody for Western Blotting When choosing a secondary antibody for western blotting, one of the main selection criteria is the species of the primary antibody that the secondary antibody binds to.
- Immobilized IP Protocol Immunoprecipitation (IP) experiments are important techniques in biochemistry and molecular biology for studying protein interactions and protein expression status.
- What is the reason for the dirty background in the WB test? How can I fix it? The Western Blot experiment can be said to be the most basic experiment in the biology major. However, although WB experiments are done every day, a normal high-definition result picture is not something you can have whenever you want.
- Western Blot FAQ Analysis Western Blot FAQ Analysis
- Introduction to the principles related to the immunoblotting experiment Western blot is used to identify large molecular weight antigens (usually proteins) that can interact with specific antibodies and to determine the size of the antigen. Proteins are first separated by SDS-PAGE and then transferred by electrophoresis to a solid support, which includes ...
- Western experimental procedure Western experimental procedure
- General Immunoprecipitation Protocol Immunoprecipitation (IP) is a molecular biology technique based on antigen-antibody specific binding, which is used to selectively separate and enrich target proteins from complex biological samples. The core of this technology is to capture the protein of interest using specific antibodies on a ...
- Histone Western Blot The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
- Protocol of Western blot for Phosphorylated Proteins Phosphoprotein western blot is a widely used technique for detecting and analyzing the phosphorylation status of proteins, which is essential for understanding various biological processes and signal transduction pathways. Some common application scenarios include signal transduction research, ...