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Goat Anti-Rabbit IgG H&L (AF546) - Secondary antibody, specific to Rabbit IgG

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Application:

IF/ICC

Host species: Goat
Reactive species: Rabbit
Isotype: Goat IgG
Conjugation: 废弃Alexa Fluor® 555. Ex: 555nm, Em: 565nm

In stock

Item Number

Ab217297

Grouped product items
SKU	Size	Availability	Price
Ab217297-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
Ab217297-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$129.90
Ab217297-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$459.90

pAb; Goat Anti-Rabbit IgG H&L Antibody (AF546); IF/ICC

View related series

AF546 (3) Goat Anti-Rabbit IgG H&L Antibody (15)

Basic Description

Product Name	Goat Anti-Rabbit IgG H&L (AF546) - Secondary antibody, specific to Rabbit IgG
Synonyms	Goat Anti-Rabbit IgG H&L Antibody \| Goat Anti Rabbit IgG H&L Antibody \| Anti-Rabbit IgG H&L Antibody \| Anti Rabbit IgG H&L Antibody \| Goat Anti-Rabbit IgG Antibody \| Goat Anti Rabbit IgG Antibody \| Anti- Rabbit IgG Antibody \| Anti Rabbit IgG Antibody
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Goat
Specificity	Rabbit IgG
Immunogen	Rabbit IgG
Conjugation	废弃Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Excitation(nm)	Green Laser(532nm)
Emission(nm)	570nm
Ex/Em(nm)	Ex:554nm;Em:570nm
Grade	ExactAb™, Validated
Product Description	Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for IF/ICC.

Product Properties

SDS-PAGE	150kDa
Purification Method	Antigen affinity purified
Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF: Goat Anti-Rabbit IgG H&L (AF546) (Ab217297)

Goat Anti-Rabbit IgG H&L (AF546) (Ab217297) - IF
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF546) (Ab217297) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IF/ICC	1/100-1/1000

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