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Recombinant AFP Antibody

    应用:
  • Capture Antibody
  • CLIA
  • ELISA
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: 27C9
  • 偶联: Unconjugated
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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab007317-10μg
10μg 现货 Stock Image
Ab007317-50μg
50μg 现货 Stock Image
Ab007317-100μg
100μg 现货 Stock Image
Ab007317-1mg
1mg 期货 Stock Image

基本信息

产品名称 Recombinant AFP Antibody
别名 甲胎蛋白抗体 | 甲胎蛋白重组单克隆抗体 | AFP抗体 | AFP重组单克隆抗体
英文别名 Alpha-1-fetoprotein | Alpha-fetoglobulin
规格或纯度 无载体, 重组, ExactAb™, 无叠氮钠, 已验证, 见COA
生化机理 Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
宿主种属 小鼠(Mouse)
特异性 AFP
应用 ELISA,CLIA
种属反应性 人(Human)
免疫原 Native protein
偶联 Unconjugated

产品属性

抗体类型 Primary antibody
克隆类型 重组抗体
克隆号 27C9
Format Whole IgG
亚型 IgG1
SDS-PAGE 150 kDa
纯化方法 Affinity purified
配对抗体 [Ab007318] AFP Mouse mAb as Detection antibody in ELISA, CLIA.
来源 CHO supernatant
物理外观 Liquid
储存缓冲液 10mM PB, 150mM NaCl, 0.1% ProClin 300, pH 7.4
防腐剂 0.1% ProClin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 长期储存-20℃(36个月);收货后建议分装,避免反复冻融。
分子类型 抗体

关联靶点(人)

AFP Tbio 甲胎蛋白(Alpha-fetoprotein) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Relevance of detection results between aladdin Antibody Pair (Ab007317 & Ab007318) and Roche in CLIA
· Capture antibody: Recombinant AFP Antibody (Ab007317) at 1.0 μg/mL.
· Detection antibody: AFP Mouse mAb (Ab007318) at 1.0 μg/mL.
· Linear Range: 0.5-1000 IU/mL, R² ≥ 0.99.

应用

应用名称 稀释比例
ELISA

1 μg/mL

CLIA

1 μg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到6个结果

批号(Lot Number) 证书类型 货号
ZJ25F0724273 分析证书 Ab007317
ZJ25F0724274 分析证书 Ab007317
ZJ25F0724275 分析证书 Ab007317
ZJ24F1011954 分析证书 Ab007317
ZJ23F0500242 分析证书 Ab007317
ZJ23F0500243 分析证书 Ab007317

技术文档和文章

组织块整体免疫组织化学染色 实验方案和技巧
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
RNA斑点杂交实验方案
RNA dot blot protocol
RNA dot blot protocol
叠氮化钠去除实验
Sodium Azide Removal Protocol

引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
3. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]
4. Yachnin, S S, Hsu, R R, Heinrikson, R L RL and Miller, J B JB..  (1977)  Studies on human alpha-fetoprotein. Isolation and characterization of monomeric and polymeric forms and amino-terminal sequence analysis..  Biochimica et biophysica acta,  (23): [PMID:70228]
5. Aoyagi, Y Y, Ikenaka, T T and Ichida, F F..  (1977)  Comparative chemical structures of human alpha-fetoproteins from fetal serum and from ascites fluid of a patient with hepatoma..  Cancer research,  [PMID:71198]
6. Aoyagi, Y Y, Ikenaka, T T and Ichida, F F..  (1978)  Copper(II)-binding ability of human alpha-fetoprotein..  Cancer research,  [PMID:80265]
7. Aoyagi, Y Y, Ikenaka, T T and Ichida, F F..  (1979)  alpha-Fetoprotein as a carrier protein in plasma and its bilirubin-binding ability..  Cancer research,  [PMID:89900]
8. Pucci, P P and 6 more authors..  (1991)  Human alpha-fetoprotein primary structure: a mass spectrometric study..  Biochemistry,  (21): [PMID:1709810]
9. Liu, M C MC, Yu, S S, Sy, J J, Redman, C M CM and Lipmann, F F..  (1985)  Tyrosine sulfation of proteins from the human hepatoma cell line HepG2..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2414772]
10. Gibbs, P E PE, Zielinski, R R, Boyd, C C and Dugaiczyk, A A..  (1987)  Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene..  Biochemistry,  (10): [PMID:2436661]
11. Sakai, M M and 5 more authors..  (1985)  The human alpha-fetoprotein gene. Sequence organization and the 5' flanking region..  The Journal of biological chemistry,  (25): [PMID:2580830]
12. Ruoslahti, E E and 5 more authors..  (1974)  Alpha fetoprotein: structure and expression in man and inbred mouse strains under normal conditions and liver injury..  Johns Hopkins medical journal. Supplement,  [PMID:4138095]
13. Beattie, W G WG and Dugaiczyk, A A..  (1982)  Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA..  Gene,  [PMID:6187626]
14. Morinaga, T T, Sakai, M M, Wegmann, T G TG and Tamaoki, T T..  (1983)  Primary structures of human alpha-fetoprotein and its mRNA..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:6192439]
15. McVey, J H JH and 6 more authors..  (1993)  A G-->A substitution in an HNF I binding site in the human alpha-fetoprotein gene is associated with hereditary persistence of alpha-fetoprotein (HPAFP)..  Human molecular genetics,  [PMID:7684942]
16. Sharony, Reuven R, Zadik, Idit I and Parvari, Ruti R..  (2004)  Congenital deficiency of alpha feto-protein..  European journal of human genetics : EJHG,  [PMID:15280901]
17. Petit, François M FM and 10 more authors..  (2009)  A new mutation in the AFP gene responsible for a total absence of alpha feto-protein on second trimester maternal serum screening for Down syndrome..  European journal of human genetics : EJHG,  [PMID:18854864]
18. Tagliabracci, Vincent S VS and 14 more authors..  (2015)  A Single Kinase Generates the Majority of the Secreted Phosphoproteome..  Cell,  (18): [PMID:26091039]

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