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Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)

    应用:
  • ELISA
  • WB
  • 宿主种属: 山羊(Goat)
  • 种属反应性: 小鼠(Mouse)
  • 亚型: Goat IgG
  • 偶联: Alkaline Phosphatase
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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab179008-10μl
10μl 现货 Stock Image
Ab179008-100μl
100μL 现货 Stock Image
Ab179008-1ml
1ml 现货 Stock Image

基本信息

产品名称 Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
别名 山羊抗小鼠IgG抗体 (AP) | 山羊抗小鼠IgG (AP) | 羊抗小鼠IgG抗体 (AP) | 羊抗小鼠IgG (AP) | 山羊抗小鼠IgG H&L 抗体 (AP) | 山羊抗小鼠IgG H&L (AP) | 羊抗小鼠IgG H&L 抗体 (AP) | 羊抗小鼠IgG H&L (AP) | 山羊抗小鼠IgG抗体,(H + L)AP共轭标记 | 碱性磷酸酶标记的山羊抗小鼠IgG H&L抗体 | 山羊抗鼠 IgG H&L(碱性磷酸酶)
英文别名 Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse Ig
规格或纯度 已验证, 见COA
宿主种属 山羊(Goat)
种属反应性 小鼠(Mouse)
交叉反应 Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原 Mouse IgG
偶联 Alkaline Phosphatase

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Goat IgG
纯化方法 Antigen affinity chromatography
物理外观 Liquid
储存缓冲液 Liquid in 50mM tris with 1mM Magnesium chloride, 0.15M NaCl, pH 8.0, containing 50% glycerol, 1% BSA and 0.05% Proclin300
防腐剂 0.05% Proclin300
浓度 见COA
储存温度 避光,-20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 储存温度为 -20°C。避免冷冻/解冻循环。避免光照。
分子类型 抗体

图片

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) at 1/20000 dilution
Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.

Predicted band size: 42 kDa
Observed band size: 42 kDa

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008)- ELISA
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well) Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was used as the secondary antibody.

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) - ELISA
Cross-reactivity of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. A serial dilution of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was used as the secondary antibody.

应用

应用名称 稀释比例
ELISA

1/1000-1/10000

WB

1:5000-1:30000

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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找到6个结果

批号(Lot Number) 证书类型 货号
ZJ25F0927351 分析证书 Ab179008
ZJ25F0927350 分析证书 Ab179008
ZJ25F0927349 分析证书 Ab179008
ZJ24F0606622 分析证书 Ab179008
ZJ24F0606621 分析证书 Ab179008
ZJ24F0606620 分析证书 Ab179008

技术文档和文章

蛋白质印迹通用实验方案
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
荧光标记抗体的蛋白质印迹法
Western Blotting with Fluorescently Labeled Antibodies
蓝色天然聚丙烯酰胺凝胶电泳实验方案 (BN-PAGE)
BN-PAGE Protocol
BN-PAGE Protocol
Western Blotting with Fluorescently Labeled Antibodies
General Protocol for Western Blotting
蛋白质印迹的二抗选择指南

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此产品的引用文献

引用文献

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936).  [10.1016/j.fbio.2024.104936]

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