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Recombinant alpha 1 Fetoprotein Antibody

    应用:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 克隆号: RM1011
  • 偶联: Unconjugated
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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab088271-10μl
10μl 现货 Stock Image
Ab088271-50μl
50μL 现货 Stock Image
Ab088271-100μl
100μL 现货 Stock Image
Ab088271-1ml
1ml 期货 Stock Image

基本信息

产品名称 Recombinant alpha 1 Fetoprotein Antibody
别名 重组甲胎蛋白抗体 | AFP抗体 | 重组甲胎蛋白抗体
英文别名 AFP | Alpha-1-fetoprotein | alpha-fetoglobulin | alpha-Fetoprotein | DSCAM2 | FETA | HP | HPAFP | Afp antibody | AFPD antibody | Alpha fetoglobulin antibody | Alpha fetoprotein antibody | Alpha fetoprotein precursor antibody | Alpha-1-fetoprotein antibody
规格或纯度 ExactAb™, Validated, Recombinant, 0.532 mg/mL
宿主种属 兔(Rabbit)
特异性 AFP
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant fragment.
阳性对照 IHC-P: Human hepatic carcinoma.
偶联 Unconjugated

产品属性

克隆类型 重组抗体
克隆号 [RM1011]
Format Whole IgG
亚型 IgG
轻链亚型 kappa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 pH: 7.2, 0.01% Sodium azide, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
防腐剂 0.01% Sodium Azide
浓度 0.532 mg/mL
储存温度 -20°C储存
运输条件 超低温冰袋运输
稳定性与储存 4°C 下运输。在 +4°C 下短期储存(1-2 周)。收货后建议分装。长期储存于 -20°C。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

AFP Tbio 甲胎蛋白(Alpha-fetoprotein) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Recombinant Anti-alpha 1 Fetoprotein Antibody (Ab088271) - IHC
Immunohistochemistry analysis of paraffin-embedded human hepatic carcinoma tissue using Recombinant Anti-alpha 1 Fetoprotein Antibody (Ab088271). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.

Recombinant Anti-alpha 1 Fetoprotein Antibody (Ab088271) - Western Blot
All lanes: Recombinant Anti-alpha 1 Fetoprotein Antibody (Ab088271) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa

应用

应用名称 稀释比例
IHC

1/100 - 1/200

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F1011818 分析证书 Ab088271
ZJ23F0700715 分析证书 Ab088271
ZJ23F0700716 分析证书 Ab088271

技术文档和文章

流式细胞术
流式细胞仪分析
如何选择和使用抗体 — 一抗
How to choose and use antibodies-primary antibody?
抗体应用和技术
Antibody applications and techniques
Antibody applications and techniques
流式细胞术实验方案
How to choose and use antibodies-primary antibody?
Antibody applications and techniques
Antibody applications and techniques
免疫沉淀(IP)裂解液和试剂
How to choose and use antibodies-primary antibody?
免疫沉淀通用实验方案
General Immunoprecipitation Protocol
General Immunoprecipitation Protocol
General Immunoprecipitation Protocol
General Immunoprecipitation Protocol
RNA免疫沉淀方案
RNA Immunoprecipitation (RIP) Protocol
RNA Immunoprecipitation (RIP) Protocol
How to choose and use antibodies-primary antibody?
固定免疫沉淀实验方案
Immobilized IP Protocol
Immobilized IP Protocol
利用甲醇通透法进行流式细胞术
Flow Cytometry Using Methanol Permeabilization
Flow Cytometry Using Methanol Permeabilization

可替换产品

引用文献

1. Yachnin, S S, Hsu, R R, Heinrikson, R L RL and Miller, J B JB..  (1977)  Studies on human alpha-fetoprotein. Isolation and characterization of monomeric and polymeric forms and amino-terminal sequence analysis..  Biochimica et biophysica acta,  (23): [PMID:70228]
2. Aoyagi, Y Y, Ikenaka, T T and Ichida, F F..  (1977)  Comparative chemical structures of human alpha-fetoproteins from fetal serum and from ascites fluid of a patient with hepatoma..  Cancer research,  [PMID:71198]
3. Aoyagi, Y Y, Ikenaka, T T and Ichida, F F..  (1978)  Copper(II)-binding ability of human alpha-fetoprotein..  Cancer research,  [PMID:80265]
4. Aoyagi, Y Y, Ikenaka, T T and Ichida, F F..  (1979)  alpha-Fetoprotein as a carrier protein in plasma and its bilirubin-binding ability..  Cancer research,  [PMID:89900]
5. Pucci, P P and 6 more authors..  (1991)  Human alpha-fetoprotein primary structure: a mass spectrometric study..  Biochemistry,  (21): [PMID:1709810]
6. Liu, M C MC, Yu, S S, Sy, J J, Redman, C M CM and Lipmann, F F..  (1985)  Tyrosine sulfation of proteins from the human hepatoma cell line HepG2..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2414772]
7. Gibbs, P E PE, Zielinski, R R, Boyd, C C and Dugaiczyk, A A..  (1987)  Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene..  Biochemistry,  (10): [PMID:2436661]
8. Sakai, M M and 5 more authors..  (1985)  The human alpha-fetoprotein gene. Sequence organization and the 5' flanking region..  The Journal of biological chemistry,  (25): [PMID:2580830]
9. Ruoslahti, E E and 5 more authors..  (1974)  Alpha fetoprotein: structure and expression in man and inbred mouse strains under normal conditions and liver injury..  Johns Hopkins medical journal. Supplement,  [PMID:4138095]
10. Beattie, W G WG and Dugaiczyk, A A..  (1982)  Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA..  Gene,  [PMID:6187626]
11. Morinaga, T T, Sakai, M M, Wegmann, T G TG and Tamaoki, T T..  (1983)  Primary structures of human alpha-fetoprotein and its mRNA..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:6192439]
12. McVey, J H JH and 6 more authors..  (1993)  A G-->A substitution in an HNF I binding site in the human alpha-fetoprotein gene is associated with hereditary persistence of alpha-fetoprotein (HPAFP)..  Human molecular genetics,  [PMID:7684942]
13. Sharony, Reuven R, Zadik, Idit I and Parvari, Ruti R..  (2004)  Congenital deficiency of alpha feto-protein..  European journal of human genetics : EJHG,  [PMID:15280901]
14. Petit, François M FM and 10 more authors..  (2009)  A new mutation in the AFP gene responsible for a total absence of alpha feto-protein on second trimester maternal serum screening for Down syndrome..  European journal of human genetics : EJHG,  [PMID:18854864]
15. Tagliabracci, Vincent S VS and 14 more authors..  (2015)  A Single Kinase Generates the Majority of the Secreted Phosphoproteome..  Cell,  (18): [PMID:26091039]
16. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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