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Goat Anti-Mouse IgG H&L (APC)

    应用:
  • Flow Cytometry
  • IF/ICC
  • 宿主种属: 山羊(Goat)
  • 种属反应性: 小鼠(Mouse)
  • 亚型: Goat IgG
  • 偶联: APC
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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab179007-100μl
100μL 现货 Stock Image
Ab179007-1ml
1ml 现货 Stock Image

基本信息

产品名称 Goat Anti-Mouse IgG H&L (APC)
别名 交联藻蓝蛋白标记山羊抗小鼠IGG H&L 抗体 | 山羊抗小鼠IgG抗体 (APC) | 羊抗小鼠IgG抗体 (APC) | 山羊抗小鼠IgG H&L 抗体 (APC) | 羊抗小鼠IgG H&L 抗体 (APC) | 山羊抗小鼠IgG抗体, (H + L) APC共轭标记 | 山羊抗小鼠IgG (APC) | 羊抗小鼠IgG (APC) | 山羊抗小鼠IgG H&L (APC) | 羊抗小鼠IgG H&L (APC) | 山羊抗鼠 IgG H&L (APC)
英文别名 Goat Anti-Mouse IgG H&L Antibody (APC) | Anti-Mouse IgG H&L Antibody (APC) | Mouse IgG H&L Antibody (APC) | APC-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (APC) | Anti-Mouse IgG Antibody (APC) | Mouse IgG Antibody (APC) | Goat ant
规格或纯度 ExactAb™, 高性能, 已验证, 无叠氮钠, Ex:650nm, Em:660nm, 1.0 mg/mL
宿主种属 山羊(Goat)
种属反应性 小鼠(Mouse)
交叉反应 Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原 Mouse IgG
偶联 APC
Excitation(nm) Red Laser(633nm)
Emission(nm) 660nm
Ex/Em(nm) Ex:650nm;Em:660nm

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Goat IgG
纯化方法 Antigen affinity purified
物理外观 Liquid
储存缓冲液 Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 1.0 mg/mL
储存温度 2-8°C储存,避光,避免反复冻融
运输条件 冰袋运输
稳定性与储存 4℃ 下保存一年。避免冷冻/解冻循环。避免光照。
分子类型 抗体

图片

Goat Anti-Mouse IgG H&L (APC) (Ab179007) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (APC) (Ab179007) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (APC) (Ab179007) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (APC) (Ab179007) at a dilution of 1/4000 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称 稀释比例
IF/ICC

1:1000

Flow Cytometry

1:100-1:2500

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批号(Lot Number) 证书类型 货号
ZJ24F0102527 分析证书 Ab179007
ZJ24F0102528 分析证书 Ab179007
ZJ25F0319074 分析证书 Ab179007
ZJ25F0319073 分析证书 Ab179007

技术文档和文章

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General Protocol for Western Blotting
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General Protocol for Western Blotting
荧光标记抗体的蛋白质印迹法
Western Blotting with Fluorescently Labeled Antibodies
Western Blotting with Fluorescently Labeled Antibodies
General Protocol for Western Blotting
免疫荧光染色实验步骤(甲醇通透法)
Immunofluorescence Staining Protocol (Methanol permeabilization)
Immunofluorescence Staining Protocol (Methanol permeabilization)
蛋白质印迹的二抗选择指南
利用甲醇通透法进行流式细胞术
Flow Cytometry Using Methanol Permeabilization
Flow Cytometry Using Methanol Permeabilization
CLARITY组织透明化染色技术
转录因子流式细胞术的固定和通透方案
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
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此产品的引用文献

引用文献

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936).  [10.1016/j.fbio.2024.104936]

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