按主题分类的文章 "Antibody"

1. Dual Cross-linking ChIP Protocol
   发布于 2025年3月28日

   26
   ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).
2. How to choose antibodies wisely?
   发布于 2024年9月12日

   1
   There are a variety of antibodies available for detecting target proteins for any purpose. In order to narrow down the choice of antibodies and select the appropriate one, the following factors need to be considered.
3. Second Antibody Selection Guide
   发布于 2024年9月12日

   1
   In immunology experiments, it is often necessary to select different secondary antibodies to meet the experimental requirements. Usually, there may be several types of secondary antibodies available for a particular experiment, and the following aspects need to be considered to select the most ...
4. Principles of Western Blot Internal Reference Antibody Selection
   发布于 2024年9月11日

   0
   There are many kinds of Western Blot internal reference antibodies, such as β-actin, β-tubulin, GAPDH, Lamin B, etc., so how should we do the selection for our own experiments? Below is a brief introduction to the principles that should be followed in selecting internal reference antibodies.
5. Immunohistochemical Antibody Selection Points
   发布于 2024年9月5日

   0
   Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.
6. Do I need to adjust the pH again after diluting EDTA Antigen Repair Solution?
   发布于 2024年8月28日

   0
   Do I need to adjust the pH again after diluting EDTA Antigen Repair Solution?
7. What are the temperature requirements for eluting antibodies in an antibody removal solution? Can the same membrane be eluted more than once?
   发布于 2024年8月28日

   0
   What are the temperature requirements for eluting antibodies in an antibody removal solution? Can the same membrane be eluted more than once?
8. How long can I keep it at -20°C? Can it be stored for a short time at 4°C or room temperature? Can it be stored at -80°C?
   发布于 2024年8月27日

   1
   How long can I keep it at -20°C? Can it be stored for a short time at 4°C or room temperature? Can it be stored at -80°C?
9. Can I still use it if I accidentally store it at room temperature for a day?
   发布于 2024年8月27日

   1
   Can I still use it if I accidentally store it at room temperature for a day?
10. Can the lysate be used without sodium aluminate?
    发布于 2024年8月27日

    1
    Can the lysate be used without sodium aluminate?