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Goat Anti-Mouse IgG H&L Antibody

Goat Anti-Mouse IgG H&L Antibody

1篇引用文献

级别和纯度:

见COA

应用:

ELISA
GICA
LF

宿主种属: 山羊(Goat)
种属反应性: 小鼠(Mouse)
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab115835-1mg	1mg	现货
Ab115835-2mg	2mg	现货
Ab115835-10mg	10mg	现货

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Goat Anti-Mouse (14)

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基本信息

产品名称	Goat Anti-Mouse IgG H&L Antibody
别名	山羊抗小鼠IgG抗体 \| 山羊抗小鼠IgG \| 山羊抗鼠IgG 抗体 \| 山羊抗鼠IgG
英文别名	Goat Anti-Mouse IgG H&L Antibody \| Goat Anti Mouse IgG H&L Antibody \| Anti-Mouse IgG H&L Antibody \| Anti Mouse IgG H&L Antibody \| Mouse IgG H&L Antibody \| Goat Anti-Mouse IgG Antibody \| Goat Anti Mouse IgG Antibody \| Anti-Mouse IgG Antibody \| Anti Mouse I
规格或纯度	无载体, ExactAb™, 已验证, 见COA
宿主种属	山羊(Goat)
应用	ELISA,LF,GICA
种属反应性	小鼠(Mouse)
交叉反应	No cross reactivity with Rabbit IgG and Human IgG.
免疫原	Mouse IgG
阳性对照	Mouse IgG
偶联	Unconjugated

产品属性

抗体类型	secondary antibody
克隆类型	多克隆抗体
Format	Whole IgG
SDS-PAGE	150kDa
纯化方法	Antigen affinity purified
来源	Serum
物理外观	Liquid
储存缓冲液	10mM PB, 150mM NaCl, 0.09% Sodium azide, pH7.4
防腐剂	0.09% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	长期储存-20℃（36个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

图片

IF: Goat Anti-Mouse IgG H&L Antibody (Ab115835)

Goat Anti-Mouse IgG H&L Antibody (Ab115835) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with biotin at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L Antibody (Ab115835)

Goat Anti-Mouse IgG H&L Antibody (Ab115835) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with FITC (Fluorescein isothiocyanate) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

应用

应用名称	稀释比例
ELISA	Use at an assay dependent concentration.
LF	Use at an assay dependent concentration
GICA	Use at an assay dependent concentration.

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找到7个结果

批号(Lot Number)	证书类型	货号
ZJ25F0521302	分析证书	Ab115835
ZJ25F0521301	分析证书	Ab115835
ZJ25F0521300	分析证书	Ab115835
ZJ23F0500203	分析证书	Ab115835
ZJ23F0500202	分析证书	Ab115835
ZJ23F0500129	分析证书	Ab115835
ZJ23F0500130	分析证书	Ab115835

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引用文献

1. Xu Tao, Yang Changwei, Lu Yang, Wang Heng, Chen Cheng, Zhou Yuchen, Chen Xiaoqing. (2024) Photocrosslinkable human amniotic membrane hydrogel for recovery from spinal cord injury. Bio-Design and Manufacturing, (1-18). [10.1007/s42242-024-00318-x]