Rabbit Anti-Mouse IgG antibody

COA

级别和纯度:

见COA

应用:

CLIA
EIA
IHC
LF
WB

宿主种属: 兔(Rabbit)
种属反应性: 小鼠(Mouse)
偶联: Unconjugated

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab170451-1mg	1mg	现货
Ab170451-5mg	5mg	现货
Ab170451-10mg	10mg	期货

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Rabbit Anti-Mouse (4)

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基本信息

产品名称	Rabbit Anti-Mouse IgG antibody
别名	兔抗鼠 IgG 抗体
规格或纯度	见COA
宿主种属	兔(Rabbit)
应用	WB,CLIA,IHC,LF,EIA
种属反应性	小鼠(Mouse)
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
纯化方法	antigen affinity chromatography
物理外观	liquid
储存缓冲液	10mM phosphate buffered saline (PBS), pH7.4
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
分子类型	抗体

图片

ELISA: Rabbit anti-mouse IgG antibody (Ab170451)

Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The plate was coated with different amounts of mouse IgG (Ab170221) (3-fold started from 100 ng/mL). Rabbit anti-mouse IgG antibody was then added at a series of different dilution ratios. Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.

ELISA: Rabbit anti-mouse IgG antibody (Ab170451)

Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Cross-reactivity of Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit anti-mouse IgG antibody was then added at different dilution ratio followed by incubation for 1h. Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
For the batch tested, Rabbit anti-mouse IgG antibody (Ab170451) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.