基本信息

产品名称	Goat Anti-Mouse IgG H&L (Biotin)
别名	山羊抗小鼠IgG抗体 (Biotin) \| 山羊抗小鼠IgG (Biotin) \| 羊抗小鼠IgG抗体 (Biotin) \| 羊抗小鼠IgG (Biotin) \| 山羊抗小鼠IgG H&L 抗体 (Biotin) \| 山羊抗小鼠IgG H&L (Biotin) \| 羊抗小鼠IgG H&L 抗体 (Biotin) \| 羊抗小鼠IgG H&L (Biotin) \| 山羊抗鼠 IgG H&L(生物素)
英文别名	Goat Anti-Mouse IgG H&L Antibody (Biotin) \| Anti-Mouse IgG H&L Antibody (Biotin) \| Mouse IgG H&L Antibody (Biotin) \| Biotin-conjugated Goat Anti-Mouse IgG (H+L) \| Goat Anti Mouse IgG Antibody (Biotin) \| Anti-Mouse IgG Antibody (Biotin) \| Mouse IgG Antibod
规格或纯度	ExactAb™, 无叠氮钠, 已验证, 高性能, 预吸附, 1.0 mg/mL
宿主种属	山羊(Goat)
种属反应性	小鼠(Mouse)
交叉反应	No cross reactivity with rabbit IgG, and human IgG.
免疫原	Mouse IgG
阳性对照	ELISA: Mouse IgG
偶联	Biotin

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,避免反复冻融
运输条件	冰袋运输
稳定性与储存	开封前储存于 4°C。为延长本产品的保质期，可加入等体积的甘油，使最终浓度约为 50%，保存在 -20°C 下。避免反复冻融循环。避光保存。
分子类型	抗体

图片

IF: Goat Anti-Mouse IgG H&L (Biotin) (Ab156253)

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

ELISA: Goat Anti-Mouse IgG H&L (Biotin) (Ab156253)

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

应用

应用名称	稀释比例
WB	1/10000-1/200000
IF/ICC	1:1000
ELISA	1/20000-1/400000

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001198	分析证书	Ab156253
ZJ23F1001199	分析证书	Ab156253

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