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Recombinant SMAD3 Antibody - Primary antibody, specific to SMAD3, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab128212
Grouped product items
SKU Size
Availability
 Price Qty
Ab128212-10μl
10μl
4
$66.90
Ab128212-50μl
50μl
2
$199.90
Ab128212-100μl
100μl
1
$297.90
Ab128212-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human SMAD3 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: SMAD3
Accession#:P84022 (4) Gene ID:4088 (6)

Basic Description

Product Name Recombinant SMAD3 Antibody - Primary antibody, specific to SMAD3, Rabbit IgG
Synonyms SMAD3 | MADH3 | Mothers against decapentaplegic homolog 3 | MAD homolog 3 | Mad3 | Mothers against DPP homolog 3 | hMAD-3 | JV15-2 | SMAD family member 3 | SMAD 3 | hSMAD3 | DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antib
Specifications & Purity ExactAb™, Validated, Recombinant, 1 mg/mL
Host species Rabbit
Specificity SMAD3
Immunogen A synthetic peptide derived from human Smad3 (AA 202-219).
Positive Control WB: Jurkat, C6, HeLa lysates. IHC: Human breast cancer tissue. ICC/IF: A549 cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SMAD3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant SMAD3 Antibody (Ab128212) - Western Blot
All lanes: Recombinant SMAD3 Antibody (Ab128212) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 51 kDa
Exposure time: 112.0 s

Recombinant SMAD3 Antibody (Ab128212) - IF
Immunocytochemistry analysis of Smad3 (green) in A549 cells using Recombinant SMAD3 Antibody (Ab128212) and cells were counterstained with DAPI (blue).

Recombinant SMAD3 Antibody (Ab128212) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant SMAD3 Antibody (Ab128212). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404795 Certificate of Analysis Apr 23, 2024 Ab128212
ZJ23F0901082 Certificate of Analysis Sep 18, 2023 Ab128212
ZJ23F0901083 Certificate of Analysis Sep 18, 2023 Ab128212

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