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SKU | Size | Availability |
Price | Qty |
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Ab186978-10μl
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10μl |
≥10
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$69.90
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Ab186978-50μl
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50μl |
2
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$199.90
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Ab186978-100μl
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100μl |
2
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$319.90
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Ab186978-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$1,999.90
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mAb (FG02/4F11); Mouse anti Human Smad3 Antibody; WB, Flow, ELISA; Unconjugated
Product Name | Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b |
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Synonyms | DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca |
Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
Host species | Mouse |
Specificity | Smad3 |
Immunogen | Recombinant full length protein corresponding to Human Smad3 |
Positive Control | WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells |
Conjugation | Unconjugated |
Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
Product Description |
Application |
Isotype | Mouse IgG2b |
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Light Chain Type | kappa |
SDS-PAGE | 150.0 KDa |
Purification Method | Protein A purified |
Concentration | 1.0 mg/mL |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 1.1 s
Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 69.1 s
Samd3 Mouse mAb (Ab186978) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Application | Dilution info |
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Flow Cytometry | 1/200-1/2000 |
WB | 1/1000 – 1/100,000 |
Find and download the COA for your product by matching the lot number on the packaging.
Lot Number | Certificate Type | Date | Item |
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Certificate of Analysis | Jun 27, 2024 | Ab186978 |
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Certificate of Analysis | Jun 27, 2024 | Ab186978 |
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Certificate of Analysis | Jun 27, 2024 | Ab186978 |
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Certificate of Analysis | Jun 14, 2024 | Ab186978 |
Starting at $66.90
Starting at $69.90
Starting at $69.90