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Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b

    Application:
  • Flow Cytometry
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: FG02/4F11
  • Conjugation: Unconjugated
In stock
Item Number
Ab186978
Grouped product items
SKU Size
Availability
Price Qty
Ab186978-10μl
10μl
≥10
$69.90
Ab186978-50μl
50μl
2
$199.90
Ab186978-100μl
100μl
2
$319.90
Ab186978-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (FG02/4F11); Mouse anti Human Smad3 Antibody; WB, Flow, ELISA; Unconjugated

Basic Description

Product Name Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b
Synonyms DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Host species Mouse
Specificity Smad3
Immunogen Recombinant full length protein corresponding to Human Smad3
Positive Control WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/1000 – 1/100,000
Flow: 1/200 - 1/2,000

Product Properties

Isotype Mouse IgG2b
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 1.1 s 

Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 69.1 s 

Samd3 Mouse mAb (Ab186978) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Application

Application Dilution info
Flow Cytometry

1/200-1/2000

WB

1/1000 – 1/100,000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0607490 Certificate of Analysis Jun 27, 2024 Ab186978
ZJ24F0607489 Certificate of Analysis Jun 27, 2024 Ab186978
ZJ24F0607488 Certificate of Analysis Jun 27, 2024 Ab186978
ZJ24R0600676 Certificate of Analysis Jun 14, 2024 Ab186978

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