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Goat Anti-Rabbit IgG H&L (AF405) - Secondary antibody, IgG

COA COA
    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: AF405
In stock
Item Number
Ab138202
Grouped product items
SKU Size
Availability
 Price Qty
Ab138202-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$87.90
Ab138202-1ml
1ml
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Goat Anti-Rabbit IgG H&L (AF405); ICC, IF, IHC, Flow, ELISA

View related series
AF405 (73) CLARITY tissue clearing technology (64) Flow Cytometry (1158) Goat Anti-Rabbit (19)

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (AF405) - Secondary antibody, IgG
Synonyms Goat Anti-Rabbit IgG H&L (AF405) | Goat Anti Rabbit IgG H&L (AF405) | Goat Anti-Rabbit IgG H&L-AF405 | Goat Anti Rabbit IgG H&L-AF405 | AF405-Goat Anti-Rabbit IgG H&L | AF405-Goat Anti Rabbit IgG H&L
Specifications & Purity ExactAb™, High Performance, Validated, Ex:401nm, Em:422nm, 1.0 mg/mL
Host species Goat
Immunogen Rabbit IgG
Conjugation AF405
Excitation(nm) Violet Laser(405nm)
Emission(nm) 422nm
Ex/Em(nm) Ex:401nm;Em:422nm
Grade Em:422nm, Ex:401nm, ExactAb™, High Performance, Validated
Product Description

Goat Anti-Rabbit IgG H&L (AF405), Secondary antibody, could be used for ICC, IF, IHC, Flow, ELISA and so on.
Background：
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 150 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective

Product Properties

Isotype IgG
Purification Method Antigen Affinity Purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Images

Goat Anti-Rabbit IgG H&L (AF405) (Ab138202) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Goat Anti-Rabbit IgG H&L (AF405) (Ab138202) was used as the secondary antibody at 1/500 dilution. Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/100-1/1000

Flow Cytometry

1/500-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0700538 Certificate of Analysis Mar 26, 2024 Ab138202
ZJ23F0700537 Certificate of Analysis Jul 06, 2023 Ab138202

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Documents & Articles

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Categories: Technical articles
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How to find the right secondary antibody?
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Immunocytochemistry (ICC) protocol
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Immunohistochemical staining protocol for frozen samples (IHC-Fr)
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RNA dot blot protocol
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Caspase Immunofluorescence Staining Protocol
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General Protocol for Western Blotting
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Western Blotting with Fluorescently Labeled Antibodies
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Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
Immunofluorescence Staining Protocol (Methanol permeabilization)
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Frozen section Immunofluorescence staining Tissue section Methanol permeability IF
Guidelines of Secondary Antibody for Western Blotting
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Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Flow Cytometry Using Methanol Permeabilization
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Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
CLARITY Tissue Clearing Staining Technology
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Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
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