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Recombinant beta Tubulin Antibody - Primary antibody, specific to TUBB, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab091197
Grouped product items
SKU Size
Availability
 Price Qty
Ab091197-10μl
10μl
4
$49.90
Ab091197-50μl
50μl
2
$147.90
Ab091197-100μl
100μl
1
$207.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human Beta Tubulin Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: TUBB
Accession#:P07437 (8) beta Tubulin (7) Gene ID:203068 (8) TUBB (7)

Basic Description

Product Name Recombinant beta Tubulin Antibody - Primary antibody, specific to TUBB, Rabbit IgG
Synonyms Beta 4 tubulin antibody | Beta 5 tubulin antibody | beta Ib tubulin antibody | Beta1 tubulin antibody | Class I beta tubulin antibody | M40 antibody | MGC117247 antibody | MGC16435 antibody | OK/SW cl.56 antibody | OK/SWcl.56 antibody | TBB5_HUMAN antibod
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity TUBB
Immunogen A synthetic peptide derived from human beta Tubulin (AA 324-337).
Positive Control WB: K562, C6, NIH3T3, HeLa, A431, Jurkat, HEK293 and MCF7 cell lysates. IHC: Human brain tissues. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Note 本产品即将售完下架，替代产品为 Ab091196
Product Description

Rabbit anti Human Beta Tubulin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/200
IF/ICC: 1/100 - 1/200
Protein Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Post-translational
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

TUBB Tclin Tubulin beta chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant beta Tubulin Antibody (Ab091197) - Western Blot
All lanes: Recombinant beta Tubulin Antibody (Ab091197) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 15.0 s


Recombinant beta Tubulin Antibody (Ab091197) - ICC/IF
Immunocytochemistry analysis of beta Tubulin(green) in HeLa cells using Recombinant beta Tubulin Antibody (Ab091197) and cells were counterstained with DAPI (blue).

Recombinant beta Tubulin Antibody (Ab091197) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Recombinant beta Tubulin Antibody (Ab091197) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IHC

1/50-1/200
IF/ICC

1/100 - 1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404259 Certificate of Analysis Apr 09, 2024 Ab091197
ZJ23F0800811 Certificate of Analysis Aug 16, 2023 Ab091197
ZJ23F0800812 Certificate of Analysis Aug 16, 2023 Ab091197

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