Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab155823-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$66.90
|
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Ab155823-50μl
|
50μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$201.90
|
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Ab155823-100μl
|
100μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$300.90
|
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Ab155823-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,500.90
|
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mAb (2F1); Mouse anti Human beta tubulin Antibody; WB, Flow, ICC / IF, ELISA; Unconjugated
| Product Name | beta Tubulin Mouse mAb - Primary antibody, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to beta tubulin, IgG3 |
|---|---|
| Synonyms | Tubulin beta-5 chain |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.8mg/mL |
| Host Species | Mouse |
| Host species | Mouse |
| Specificity | beta tubulin |
| Application | WB,Flow,ELISA,ICC/IF |
| Immunogen | Recombinant human beta tubulin |
| Positive Control | WB: HeLa, A431, HEK293, MCF7, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Monoclonal antibodies |
| Isotype | IgG3 |
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | Hybridoma supernatant |
| Shape | Liquid |
| Concentration | 0.8mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
beta Tubulin Mouse mAb (Ab155823) - Western Blot
All lanes: beta Tubulin Mouse mAb (Ab155823) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
beta Tubulin Mouse mAb (Ab155823) - ICC/IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Tubulin Mouse mAb (Ab155823) - Flow Cytometry
Flow analysis of beta Tubulin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
| Application | Dilution info |
|---|---|
| IF/ICC |
0.5 μg/mL
|
| Flow Cytometry |
0.016-2.0μg/mL
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 14, 2023 | Ab155823 | |
| Certificate of Analysis | Aug 14, 2023 | Ab155823 |
Starting at $59.90
Starting at $66.90
Starting at $49.90
Starting at $66.90