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beta Tubulin Mouse mAb - Primary antibody, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to beta tubulin, IgG3

    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG3
  • Clone Number: CD01/2F1
  • Conjugation: Unconjugated
In stock
Item Number
Ab155823
Grouped product items
SKU Size
Availability
Price Qty
Ab155823-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab155823-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$201.90
Ab155823-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$300.90
Ab155823-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,500.90

mAb (2F1); Mouse anti Human beta tubulin Antibody; WB, Flow, ICC / IF, ELISA; Unconjugated

Basic Description

Product Name beta Tubulin Mouse mAb - Primary antibody, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to beta tubulin, IgG3
Synonyms Tubulin beta-5 chain
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.8mg/mL
Host Species Mouse
Host species Mouse
Specificity beta tubulin
Application WB,Flow,ELISA,ICC/IF
Immunogen Recombinant human beta tubulin
Positive Control WB: HeLa, A431, HEK293, MCF7, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/2,000-1/100,000; Predicted molecular weight: 50 kDa
Flow: 0.016-2μg/mL
ICC/IF: 0.5μg/mL

Product Properties

Antibody Type Primary antibody
Clonality Monoclonal antibodies
Isotype IgG3
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Source Hybridoma supernatant
Shape Liquid
Concentration 0.8mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

TUBB Tclin Tubulin beta chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

beta Tubulin Mouse mAb (Ab155823) - Western Blot
All lanes: beta Tubulin Mouse mAb (Ab155823) at 1/10000 dilution


Samples: Lysates at 20 µg per lane


Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution


Predicted band size: 49 kDa
Observed band size: 50 kDa

beta Tubulin Mouse mAb (Ab155823) - ICC/IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

beta Tubulin Mouse mAb (Ab155823) - Flow Cytometry
Flow analysis of beta Tubulin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Application

Application Dilution info
IF/ICC

0.5 μg/mL

Flow Cytometry

0.016-2.0μg/mL

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800804 Certificate of Analysis Aug 14, 2023 Ab155823
ZJ23F0800803 Certificate of Analysis Aug 14, 2023 Ab155823

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