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Recombinant Rad21 Antibody - Primary antibody, specific to RAD21, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Hamster,Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab124351
Grouped product items
SKU Size
Availability
 Price Qty
Ab124351-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab124351-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab124351-100μl
100μl
1
$297.90
Ab124351-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,307.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human Rad21 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: RAD21
Accession#:O60216 (1) Gene ID:5885 (1) RAD21 (1)

Basic Description

Product Name Recombinant Rad21 Antibody - Primary antibody, specific to RAD21, Rabbit IgG
Synonyms CDLS4 antibody | Double-strand-break repair protein rad21 homolog antibody | hHR21 antibody | HR21 antibody | HRAD21 antibody | KIAA0078 antibody | MCD1 antibody | Nuclear matrix protein 1 antibody | NXP-1 antibody | NXP1 antibody | Protein involved in DN
Specifications & Purity ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species Rabbit
Specificity RAD21
Immunogen A synthetic peptide derived from human Rad21 (AA 600-631).
Positive Control WB: Hela, A549, K562 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cell.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Rad21 Antibody, Recombinant, could be used for  WB, IHC, ICC, IF and so on.

Application:

· WB: 1/500-1/1000

· IHC: 1/50-1/100 
· ICC/IF: 1/50-1/200

Protein Function：

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kD
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.6 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RAD21 Tbio Double-strand-break repair protein rad21 homolog (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant Rad21 Antibody (Ab124351) - Western Blot
All lanes: Recombinant Rad21 Antibody (Ab124351) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 130 kDa
Observed band size: 117 kDa
Exposure time: 5.8 s 

Recombinant Rad21 Antibody (Ab124351) - ICC/IF
Immunocytochemistry analysis of Rad21 (green) in HeLa cells using Recombinant Rad21 Antibody (Ab124351) and cells were counterstained with DAPI (blue).

Recombinant Rad21 Antibody (Ab124351) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Rad21 Antibody (Ab124351). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800849 Certificate of Analysis Aug 17, 2023 Ab124351
ZJ23F0800850 Certificate of Analysis Aug 17, 2023 Ab124351

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