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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab124343-10μl
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10μl |
2
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$69.90
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Ab124343-50μl
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50μl |
1
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$199.90
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Ab124343-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
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Ab124343-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,999.90
|
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Recombinant; Rabbit anti Human RAD18 Antibody; WB; Unconjugated
| Product Name | Recombinant RAD18 Antibody - Primary antibody, specific to RAD18, Rabbit IgG |
|---|---|
| Synonyms | RNF73 | 2810024C04Rik antibody | DNA repair protein rad18 antibody | E3 ubiquitin-protein ligase RAD18 antibody | EC 6.3.2.- antibody | hHR 18 antibody | hHR18 antibody | hRAD 18 antibody | hRAD18 antibody | MGC156682 antibody | Postreplication repair E3 |
| Specifications & Purity | Recombinant, ExactAb™, Validated, High Performance, See COA |
| Host species | Rabbit |
| Specificity | RAD18 |
| Immunogen | A synthetic peptide derived from human RAD18 (AA 50-100) |
| Positive Control | WB: HeLa, MCF7, A431, A549, K562, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Rabbit anti Human RAD18 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/10000-1/20000 Protein Function: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant RAD18 Antibody (Ab124343) - Western Blot
All lanes: Recombinant RAD18 Antibody (Ab124343) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 69 kDa. The extra bands (120 kDa) may represent modified products (ubiquitination, phosphorylation).
Exposure time: 60.0 s
| Application | Dilution info |
|---|---|
| WB | 1/10000 - 1/20000 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jun 24, 2024 | Ab124343 | |
| Certificate of Analysis | Jun 24, 2024 | Ab124343 |