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Recombinant PP2A alpha/beta Antibody - Primary antibody, specific to PPP2CA/PPP2CB, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Hamster,Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab122709
Grouped product items
SKU Size
Availability
 Price Qty
Ab122709-10μl
10μl
5
$66.90
Ab122709-50μl
50μl
2
$199.90
Ab122709-100μl
100μl
1
$297.90
Ab122709-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human PP2A alpha/beta Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: PPP2CA Click to view target information: PPP2CB
Accession#:P62714 (1) Accession#:P67775 (2) Gene ID:5515 (2) Gene ID:5516 (1) PP2A alpha/beta (1) PPP2CA (2) PPP2CB (1)

Basic Description

Product Name Recombinant PP2A alpha/beta Antibody - Primary antibody, specific to PPP2CA/PPP2CB, Rabbit IgG
Synonyms PPP2CA | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | PP2A-alpha | Replication protein C | RP-C | PP2A A antibody | PP2A alpha antibody | PP2A B antibody | PP2A beta antibody | PP2A-alpha antibody | PP2A-beta antibody | PP2AA_
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity PPP2CA/PPP2CB
Immunogen A synthetic peptide derived from human PP2A alpha/beta (AA 277-294).
Positive Control WB: HeLa, C6, NIH/3T3, A431, Jurkat, HEK293, A549 and Raw264.7 cell lysates. IHC: Human lung cancer. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PP2A alpha/beta Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PPP2CA Tchem Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
PPP2CB Tbio Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
PPP2CB Tbio Serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform (1 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant PP2A alpha/beta Antibody (Ab122709) - Western Blot
All lanes: Recombinant PP2A alpha/beta Antibody (Ab122709) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 34 kDa
Exposure time: 10.0 s

Recombinant PP2A alpha/beta Antibody (Ab122709) - IF
Immunocytochemistry analysis of PP2A alpha/beta (green) in HeLa cells using Recombinant PP2A alpha/beta Antibody (Ab122709) and cells were counterstained with DAPI (blue).

Recombinant PP2A alpha/beta Antibody (Ab122709) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant PP2A alpha/beta Antibody (Ab122709). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901067 Certificate of Analysis Sep 18, 2023 Ab122709
ZJ23F0901066 Certificate of Analysis Sep 18, 2023 Ab122709

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