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Recombinant MCM6 Antibody - Primary antibody, specific to MCM6, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab114562
Grouped product items
SKU Size
Availability
 Price Qty
Ab114562-10μl
10μl
2
$69.90
Ab114562-50μl
50μl
1
$209.90
Ab114562-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$509.90
Ab114562-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,819.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human MCM6 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: MCM6
Accession#:Q14566 (1) Gene ID:4175 (1) MCM6 (1)

Basic Description

Product Name Recombinant MCM6 Antibody - Primary antibody, specific to MCM6, Rabbit IgG
Synonyms EC 3.6.4.12 | p105MCM | DNA replication licensing factor MCM6 antibodyMCG40308 antibody | Mcm6 antibody | MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody | MCM6 minichromosome maintenance deficient 6 antibody | MCM6_HUMAN ant
Specifications & Purity ExactAb™, Validated, Recombinant, 0.8 mg/mL
Host species Rabbit
Specificity MCM6
Immunogen Recombinant protein of human MCM6 (AA 676-821)
Positive Control WB: Jurkat, HeLa, MCF7, U2OS, Hep G2, A431, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: Hela cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human MCM6 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.8 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MCM6 Tbio DNA replication licensing factor MCM6 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant MCM6 Antibody (Ab114562) - Western Blot
All lanes: Recombinant MCM6 Antibody (Ab114562) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 93 kDa
Observed band size: 97 kDa
Exposure time: 50.8 s

Recombinant MCM6 Antibody (Ab114562) - IF/ICC
Immunocytochemistry analysis of MCM6 (green) in HeLa using Recombinant MCM6 Antibody (Ab114562), and cells were counterstained with DAPI (blue).

Recombinant MCM6 Antibody (Ab114562) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM6 Antibody (Ab114562). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0303902 Certificate of Analysis Apr 02, 2024 Ab114562
ZJ24F0404053 Certificate of Analysis Apr 02, 2024 Ab114562

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