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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab099931-10μl
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10μl |
2
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$66.90
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Ab099931-50μl
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50μl |
1
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$199.90
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Ab099931-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$299.90
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Ab099931-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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Recombinant; Rabbit anti Human DKC1/Dyskerin Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant DKC1/Dyskerin Antibody - Primary antibody, specific to DKC1, Rabbit IgG |
|---|---|
| Synonyms | CBF5 | CBF5 homolog | Cbf5p homolog | DKC 1 | DKC | Dkc1 | DKC1_HUMAN | DKCX | Dyskeratosis congenita 1 | Dyskeratosis congenita 1 dyskerin | Dyskerin | H/ACA ribonucleoprotein complex subunit 4 | NAP 57 | NAP57 | NAP-57 | NOLA 4 | NOLA4 | Nopp140 associa |
| Specifications & Purity | Recombinant, ExactAb™, Validated, High Performance, See COA |
| Biochemical and Physiological Mechanisms | Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such |
| Host species | Rabbit |
| Specificity | DKC1 |
| Immunogen | A synthetic peptide derived from human DKC1 (AA 371-384) |
| Positive Control | WB: HeLa, MCF7 and U2OS cell lysates. IHC: Human breast cancer tissue. ICC/IF: K562 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Rabbit anti Human DKC1/Dyskerin Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant DKC1/Dyskerin Antibody (Ab099931) - Western Blot
All lanes: Recombinant DKC1/Dyskerin Antibody (Ab099931) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 45, 56 kDa
Exposure time: 79.4 s
Recombinant DKC1/Dyskerin Antibody (Ab099931) - IF/ICC
Immunocytochemistry analysis of DKC1 (green) in K562 using Recombinant DKC1/Dyskerin Antibody (Ab099931), and cells were counterstained with DAPI (blue).
Recombinant DKC1/Dyskerin Antibody (Ab099931) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant DKC1/Dyskerin Antibody (Ab099931). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/100-1/200 |
| WB | 1/1000 - 1/5000 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Mar 14, 2024 | Ab099931 | |
| Certificate of Analysis | Mar 14, 2024 | Ab099931 |