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Recombinant Cdk9 Antibody - Primary antibody, specific to Cdk9, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab095812
Grouped product items
SKU Size
Availability
 Price Qty
Ab095812-10μl
10μl
2
$66.90
Ab095812-50μl
50μl
1
$199.90
Ab095812-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab095812-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human Cdk9 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: CDK9
Accession#:P50750 (1) CDK9 (1) Gene ID:1025 (1)

Basic Description

Product Name Recombinant Cdk9 Antibody - Primary antibody, specific to Cdk9, Rabbit IgG
Synonyms C-2K antibody | CDC2 related kinase antibody | CDC2L4 antibody | Cdk 9 antibody | Cdk9 antibody | CDK9_HUMAN antibody | Cell division cycle 2-like protein kinase 4 antibody | Cell division protein kinase 9 antibody | CTK1 antibody | Cyclin dependent kinas
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity Cdk9
Immunogen A synthetic peptide derived from human Cdk9 (AA 360-372)
Positive Control WB: K562, HeLa, MCF7, Hep G2, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissues. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Cdk9 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CDK9 Tchem Cyclin-dependent kinase 9 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
CDK9 Tchem Cyclin-dependent kinase 9 (2495 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CDK9 Tchem CDK9/Cyclin K (143 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CDK9 Tchem CDK9/Cyclin-T2 (7 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant Cdk9 Antibody (Ab095812) - Western Blot
All lanes: Recombinant Cdk9 Antibody (Ab095812) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 40 kDa
Exposure time: 21.0 s

Recombinant Cdk9 Antibody (Ab095812) - IF/ICC
Immunocytochemistry analysis of Cdk9 (green) in HeLa using Recombinant Cdk9 Antibody (Ab095812), and cells were counterstained with DAPI (blue).

Recombinant Cdk9 Antibody (Ab095812) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Cdk9 Antibody (Ab095812). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0303162 Certificate of Analysis Mar 13, 2024 Ab095812
ZJ24F0303161 Certificate of Analysis Mar 13, 2024 Ab095812

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