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Recombinant AMPK alpha 1 Antibody - Primary antibody, specific to PRKAA1, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab088655
Grouped product items
SKU Size
Availability
 Price Qty
Ab088655-10μl
10μl
9
$59.90
Ab088655-50μl
50μl
2
$179.90
Ab088655-100μl
100μl
1
$279.90
Ab088655-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,249.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human AMPK alpha 1 Antibody; WB, IHC, IF/ICC; Unconjugated

View related series
Click to view target information: PRKAA1
Accession#:Q13131 (2) AMPK alpha 1 (2) Gene ID:5562 (2) PRKAA1 (2)

Basic Description

Product Name Recombinant AMPK alpha 1 Antibody - Primary antibody, specific to PRKAA1, Rabbit IgG
Synonyms 5 AMP activated protein kinase alpha 1catalytic subunit antibody | 5 AMP activated protein kinase catalytic alpha 1 chain antibody | 5' AMP activated protein kinase catalytic subunit alpha 1 antibody | 5'-AMP-activated protein kinase catalytic subunit alp
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears
Host species Rabbit
Specificity PRKAA1
Immunogen A synthetic peptide derived from human AMPK alpha 1 (AA 500-559).
Positive Control WB: Raw264.7, MCF7, C6, K562, Hep G2, NIH/3T3 and HeLa cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human AMPK alpha 1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. 

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PRKAA1 Tclin 5'-AMP-activated protein kinase catalytic subunit alpha-1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant AMPK alpha 1 Antibody (Ab088655) - Western Blot
All lanes: Recombinant AMPK alpha 1 Antibody (Ab088655) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 66 kDa
Exposure time: 5.0 s

Recombinant AMPK alpha 1 Antibody (Ab088655) - IF/ICC
Immunocytochemistry analysis of AMPK alpha 1 (green) in HeLa cells using Recombinant AMPK alpha 1 Antibody (Ab088655), and cells were counterstained with DAPI (blue).

Recombinant AMPK alpha 1 Antibody (Ab088655) - IHC
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Recombinant AMPK alpha 1 Antibody (Ab088655). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
IHC

1/100 - 1/200
WB

1/1000 - 1/2000
IF/ICC

1/50 - 1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404137 Certificate of Analysis Apr 03, 2024 Ab088655
ZJ23F1001412 Certificate of Analysis Oct 26, 2023 Ab088655
ZJ23F1001413 Certificate of Analysis Oct 26, 2023 Ab088655

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