Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab120985-10μl
|
10μl |
2
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$69.90
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Ab120985-50μl
|
50μl |
1
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$199.90
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Ab120985-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$299.90
|
|
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Ab120985-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,499.90
|
|
Recombinant; Rabbit anti Human PDK1 Antibody; WB; Unconjugated
| Product Name | Recombinant PDK1 Antibody - Primary antibody, specific to PDK1, Rabbit IgG |
|---|---|
| Synonyms | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody | HGNC:8809 antibody | Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody | PDH kinase 1 antibody | Pdk1 antibody | PDK1_HUMAN antibody | Pyruvate dehydrogenase ki |
| Specifications & Purity | Recombinant, ExactAb™, Validated, KD Validation, See COA |
| Biochemical and Physiological Mechanisms | Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. |
| Host species | Rabbit |
| Specificity | PDK1 |
| Immunogen | A synthetic peptide derived from human PDK1 (AA 15-64) |
| Positive Control | WB: LNCaP, HeLa, Hep G2, Jurkat, A431, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, KD Validation, Recombinant, Validated |
| Product Description |
Rabbit anti Human PDK1 Antibody, Recombinant, could be used for WB and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant PDK1 Antibody (Ab120985) - Western Blot
All lanes: Recombinant PDK1 Antibody (Ab120985) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 45, 70 kDa. The molecular weight observed is consistent with what has been described in PMID: 30171166.
Exposure time: 80.0 s
Recombinant PDK1 Antibody (Ab120985) - Western Blot
All lanes use Recombinant PDK1 Antibody (Ab120985) at 1/1000 dilution for 1 hour at room temperature.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jul 25, 2024 | Ab120985 | |
| Certificate of Analysis | Jul 25, 2024 | Ab120985 |