Basic Description

Product Name	Recombinant GAPDH Antibody - Primary antibody, specific to GAPDH, Rabbit IgG
Synonyms	38 kDa BFA-dependent ADP-ribosylation substrate antibody \| aging associated gene 9 protein antibody \| Aging-associated gene 9 protein antibody \| BARS-38 antibody \| cb609 antibody \| EC 1.2.1.12 antibody \| Epididymis secretory sperm binding protein Li 162eP
Specifications & Purity	ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
Host species	Rabbit
Specificity	GAPDH
Immunogen	A synthetic peptide corresponding to residues of human GAPDH was used as an immunogen (AA 200-300).
Positive Control	WB: A431, MCF-7, A549, HEK293, HeLa, Raji, Jurkat, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Prostate cancer tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate.
Conjugation	Unconjugated
Grade	ExactAb™, High Performance, Recombinant, Validated
Product Description	Rabbit anti Human GAPDH Antibody, Recombinant (RR688), could be used for WB, IHC, Flow, IF, ICC, IP and so on. Application: WB: 1/5000 - 1/40000 IHC: 1/1600 - 1/3200 Flow: 1/200 - 1/1000 IF/ICC: 1/2000 - 1/10000 IP: 1/50 Protein Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Post-translational S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)

Discover Target: GAPDH

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104788) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 1.0 s

IF: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IF
IF analysis of GAPDH (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant GAPDH Antibody (Ab104788) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

IHC: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of prostate cancer tissue labelling GAPDH with Recombinant GAPDH Antibody (Ab104788) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant GAPDH Antibody (Ab104788) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant GAPDH Antibody (Ab104788) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

IP: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IP
GAPDH was immunoprecipitated from 0.1 mg of HeLa whole cell lysate with Recombinant GAPDH Antibody (Ab104788) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant GAPDH Antibody (Ab104788) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant GAPDH Antibody (Ab104788) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 2 μg (input).

Application

Application	Dilution info
IP	1/50
Flow Cytometry	1/200 - 1/1000
IF/ICC	1/2000 - 1/10000
WB	1/5000 - 1/40000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505915	Certificate of Analysis	May 21, 2024	Ab104788
ZJ23F0901096	Certificate of Analysis	Sep 18, 2023	Ab104788
ZJ23F0901097	Certificate of Analysis	Sep 18, 2023	Ab104788