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GAPDH Mouse mAb (Biotin) - Primary antibody, specific to GAPDH

    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: B08/4A11
  • Conjugation: Biotin
In stock
Item Number
Ab219252
Grouped product items
SKU Size
Availability
Price Qty
Ab219252-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab219252-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab219252-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab219252-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (B08/4A11); Mouse anti Human GAPDH Antibody (Biotin); WB; IF/ICC; Flow Cytometry

Basic Description

Product Name GAPDH Mouse mAb (Biotin) - Primary antibody, specific to GAPDH
Synonyms 38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Mouse
Specificity GAPDH
Immunogen Recombinant full length human GAPDH
Positive Control WB: HEK293, HeLa, Jurkat and K562 whole cell lysates Flow Cytometry: HEK293 cells ICC/IF: HeLa cells
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Mouse anti Human GAPDH Antibody, Monoclonal (B08/4A11), could be used for WB; IF/ICC and Flow Cytometry.

Product Properties

Light Chain Type kappa
Purification Method Protein A purified
Concentration 0.5 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Associated Targets(Human)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

GAPDH Mouse mAb (Biotin) (Ab219252) - Western Blot
All lanes: GAPDH Mouse mAb (Biotin) (Ab219252) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 127.5 s

GAPDH Mouse mAb (Biotin) (Ab219252) - Flow Cytometry
Flow cytometry analysis of GAPDH expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with GAPDH Mouse mAb (Biotin) (Ab219252) (Red). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

GAPDH Mouse mAb (Biotin) (Ab219252) – IF/ICC
IF analysis of GAPDH (green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Biotin) (Ab219252) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/100-1/1000

Flow Cytometry

1/1000 - 1/10000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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