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GAPDH Mouse mAb - Primary antibody, specific to GAPDH, IgG1

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: B08/4A11
  • Conjugation: Unconjugated
In stock
Item Number
Ab155825
Grouped product items
SKU Size
Availability
 Price Qty
Ab155825-10μl
10μl
4
$49.90
Ab155825-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$149.90
Ab155825-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$219.90
Ab155825-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,699.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (B08/4A11); Mouse anti Human GAPDH Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: GAPDH
Accession#:P04406 (7) Flow Cytometry (1158) GAPDH (7) Gene ID:2597 (7)

Basic Description

Product Name GAPDH Mouse mAb - Primary antibody, specific to GAPDH, IgG1
Synonyms 38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, 1.0 mg/mL
Host species Mouse
Specificity GAPDH
Immunogen Recombinant full length human GAPDH
Positive Control WB: HEK293, HeLa, Jurkat, A431, HepG2 and K562 whole cell lysates; Flow: Jurkat cells; ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/1,000-1/100,000 ; Predicted molecular weight: 37 kDa
Flow: 0.016 μg/mL-1 μg/mL
ICC/IF: 0.5 μg/mL
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. This GAPDH antibody can be used as a loading control antibody.

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

GAPDH Mouse mAb (Ab155825) - Western Blot
All lanes: GAPDH Antibody (Ab155825) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 35.8 kDa
Observed band size: 35.6 kDa
Exposure time: 36.9 s 

GAPDH Mouse mAb (Ab155825) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with GAPDH Mouse mAb (Ab155825) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.

GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL and Recombinant COX IV Antibody (Ab097410) (Red) at 1μg/mL in blocking buffer for 2h at room temperature and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 and Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147) for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

0.5 μg/mL

WB

1/1000-1/100000 ; Predicted molecular weight: 37 kDa
Flow Cytometry

0.016 μg/mL- 1 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ25R0402363 Certificate of Analysis Apr 18, 2025 Ab155825
ZJ24F0404297 Certificate of Analysis Apr 09, 2024 Ab155825
ZJ23F1001304 Certificate of Analysis Oct 16, 2023 Ab155825
ZJ23F1001305 Certificate of Analysis Oct 16, 2023 Ab155825

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