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Recombinant EpCAM Antibody - Primary antibody, specific to EpCAM, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Clone Number: RR694
  • Conjugation: Unconjugated
In stock
Item Number
Ab101839
Grouped product items
SKU Size
Availability
 Price Qty
Ab101839-10μl
10μl
5
$59.90
Ab101839-50μl
50μl
1
$177.90
Ab101839-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab101839-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,397.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR694); Rabbit anti Human EpCAM Antibody; WB, IHC, Flow, IP; Unconjugated

View related series
Click to view target information: EPCAM
Accession#:P16422 (16) EPCAM (19) Flow Cytometry (1158) Gene ID:4072 (17)

Basic Description

Product Name Recombinant EpCAM Antibody - Primary antibody, specific to EpCAM, Rabbit IgG
Synonyms 17 1A antibody | 323/A3 antibody | Adenocarcinoma associated antigen antibody | Adenocarcinoma-associated antigen antibody | Antigen identified by monoclonal antibody AUA1 antibody | AUA1 antibody | CD326 antibody | CD326 antigen antibody | Cell surface g
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
Host species Rabbit
Specificity EpCAM
Immunogen A synthetic peptide corresponding to residues of human EPCAM. (AA 1-100)
Positive Control WB: Hep G2, HEK293 and A431 cell lysates. IHC: Human Uterus tissue. Flow: HCT-116 cells. IP: HT-29 whole cell lysate. IF/ICC: HT-29 cells.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human EpCAM Antibody, Recombinant antibody (RR694), could be used for WB, IHC, Flow and IP.
Application:
WB: 1/2000 – 1/5000
IHC: 1/1600-1/3200
Flow: 1/800-1/2000
IP: 1/50

IF/ICC: 1/1000 - 1/2000
Protein Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

EPCAM Tbio Epithelial cell adhesion molecule (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant EpCAM Antibody (Ab101839) - Western Blot
All lanes: Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 5.0 s

Recombinant EpCAM Antibody (Ab101839) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling EpCAM with Recombinant EpCAM Antibody (Ab101839) at 1/3200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant EpCAM Antibody (Ab101839) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained with Recombinant EpCAM Antibody (Ab101839) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant EpCAM Antibody (Ab101839) ( 1/800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

Recombinant EpCAM Antibody (Ab101839) - IP
EpCAM was immunoprecipitated from 0.4 mg of HT-29 whole cell lysate with Recombinant EpCAM Antibody (Ab101839) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant EpCAM Antibody (Ab101839) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant EpCAM Antibody (Ab101839) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)

Exposure: 120 s

Application

Application Dilution info
IP

1/50

Flow Cytometry

1/800 - 1/2000
WB

1/2000 - 1/5000

IF/ICC

1/1000 - 1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901018 Certificate of Analysis Sep 11, 2023 Ab101839
ZJ23F0901017 Certificate of Analysis Sep 11, 2023 Ab101839

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Documents & Articles

Flow Cytometry
Categories: Technical articles
Tags:
Flow Cytometry
Flow cytometry analysis
Categories: Technical articles
Tags:
Forward scatter Side scatter Dichroic filters/mirrors The multicolor flow cytometer
Flow cytometry protocol
Categories: Protocols
Tags:
Antibodies Flow Cytometry Flow Antibodies Reactive dyes Indirect antibody labelling
Immunoprecipitation (IP) lysates and reagents
Categories: Protocols
Tags:
Immunoprecipitation lysate Tissue lysate
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies
Immunohistochemistry (IHC) staining antigen retrieval protocol
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval
General Immunoprecipitation Protocol
Categories: Protocols
Tags:
Immunoprecipitation Western blot Cell lysate Co-immunoprecipitation IP Protein interaction Protein A/G magnetic beads
RNA Immunoprecipitation (RIP) Protocol
Categories: Protocols
Tags:
IP Protein A/G magnetic beads RNA immunoprecipitation RIP RNA protein interaction Isotype control antibody Input Crosslinking
Immobilized IP Protocol
Categories: Protocols
Tags:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
Flow Cytometry Using Methanol Permeabilization
Categories: Protocols
Tags:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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