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ATG7 Antibody - Primary antibody, specific to ATG7, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab090020
Grouped product items
SKU Size
Availability
Price Qty
Ab090020-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab090020-50μl
50μl
1
$189.90
Ab090020-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab090020-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

pAb; Rabbit anti Human ATG7 Antibody; WB, IHC; Unconjugated

Basic Description

Product Name ATG7 Antibody - Primary antibody, specific to ATG7, Rabbit IgG
Synonyms 1810013K23Rik antibody | Apg 7 antibody | APG7 autophagy 7 like antibody | APG7 autophagy 7-like (S. cerevisiae)antibody | APG7 like antibody | APG7, S. cerevisiae, homolog of antibody | APG7-like antibody | APG7L antibody | ATG 7 antibody | ATG12-activat
Specifications & Purity ExactAb™, Validated, 0.6 mg/mL
Host species Rabbit
Specificity ATG7
Immunogen A synthetic peptide derived from human ATG7 (AA 691-703)
Positive Control WB: Hep G2, THP-1, HeLa, Jurkat and HEK293 cell lysates. IHC: Human colon tissue.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Rabbit anti Human ATG7 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB:  1/500-1/1000

IHC: 1/50-1/100

Protein Function:

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.  

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.6 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ATG7 Tchem Ubiquitin-like modifier-activating enzyme ATG7 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

ATG7 Antibody (Ab090020) - Western Blot
All lanes: ATG7 Antibody (Ab090020) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 74 kDa
Exposure time: 36.9 s

ATG7 Antibody (Ab090020) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 Antibody (Ab090020). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1202237 Certificate of Analysis Dec 26, 2023 Ab090020
ZJ23F1202238 Certificate of Analysis Dec 26, 2023 Ab090020

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