Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab090020-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
|
|
Ab090020-50μl
|
50μl |
1
|
$189.90
|
|
|
Ab090020-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
|
|
|
Ab090020-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,599.90
|
|
pAb; Rabbit anti Human ATG7 Antibody; WB, IHC; Unconjugated
| Product Name | ATG7 Antibody - Primary antibody, specific to ATG7, Rabbit IgG |
|---|---|
| Synonyms | 1810013K23Rik antibody | Apg 7 antibody | APG7 autophagy 7 like antibody | APG7 autophagy 7-like (S. cerevisiae)antibody | APG7 like antibody | APG7, S. cerevisiae, homolog of antibody | APG7-like antibody | APG7L antibody | ATG 7 antibody | ATG12-activat |
| Specifications & Purity | ExactAb™, Validated, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | ATG7 |
| Immunogen | A synthetic peptide derived from human ATG7 (AA 691-703) |
| Positive Control | WB: Hep G2, THP-1, HeLa, Jurkat and HEK293 cell lysates. IHC: Human colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Rabbit anti Human ATG7 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
ATG7 Antibody (Ab090020) - Western Blot
All lanes: ATG7 Antibody (Ab090020) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 74 kDa
Exposure time: 36.9 s
ATG7 Antibody (Ab090020) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 Antibody (Ab090020). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Dec 26, 2023 | Ab090020 | |
| Certificate of Analysis | Dec 26, 2023 | Ab090020 |