按主题分类的文章 "Western blotting"

1. Protocol of Western Blotting For High Molecular Weights
   发布于 2024年10月30日

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   High molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.
2. Immunoprecipitation Protocol for Native Proteins
   发布于 2024年10月23日

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   Immunoprecipitation of natural proteins is a classic method for studying natural protein interactions, which relies on the specific binding between antibodies and proteins (antigens). This technique can be used to qualitatively detect proteins, determine the physiological role of proteins in ...
3. BN-PAGE Protocol
   发布于 2024年9月25日

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   Blue native polyacrylamide gel electrophoresis (BN-PAGE) is an electrophoretic technique used to separate and analyze large protein complexes. It preserves protein complexes in their native state under non-denaturing conditions by treating samples with mild detergents and negatively charged dyes ...
4. Methods of Lysate Preparation for Western Blotting
   发布于 2024年9月11日

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   This method provides a recipe of lysate and buffer preparation for lysing cells or tissues for western blot analysis.
5. General Protocol for Western Blotting
   发布于 2024年9月10日

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   Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
6. Guidelines of Primary Antibodies for Western Blotting
   发布于 2024年7月12日

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   Polyclonal, monoclonal, and recombinant antibodies are all suitable for western blotting. Polyclonal antibodies are a collection of many monoclonal antibodies that can vary between immunizations and batches. Polyclonal antibodies recognize multiple epitopes of an antigen and are therefore ...
7. Loading Controls for Western Blotting
   发布于 2023年11月8日

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   Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.
8. Stripping and Reprobing Western Blotting Membranes
   发布于 2022年9月5日

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   免疫印迹是研究蛋白质功能和定位的常用技术。通常，蛋白质样品可在SDS-PAGE上分离并转印至硝酸纤维素膜(NC膜)或PVDF膜上，然后用特异性抗体进行检测。与可重复利用Southern和Northern印迹的核酸技术不同，免疫印迹很难重复使用。 ...