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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab133968-10μl
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10μl |
2
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$66.90
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Ab133968-50μl
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50μl |
1
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$189.90
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Ab133968-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
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Ab133968-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,809.90
|
|
Recombinant; Rabbit anti Human VCAM1 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | VCAM1 Antibody - Primary antibody, specific to VCAM1, Rabbit IgG |
|---|---|
| Synonyms | CD106 antibody | CD106 Antigen antibody | INCAM 100antibody | INCAM-100 antibody | L1CAM antibody | MGC99561 antibody | V-CAM 1 antibody | Vascular Cell Adhesion Molecule 1 antibody | Vascular cell adhesion protein 1 antibody | VCAM 1 antibody | VCAM-1 an |
| Specifications & Purity | ExactAb™, Validated, Recombinant, See COA |
| Biochemical and Physiological Mechanisms | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiolog |
| Host species | Rabbit |
| Specificity | VCAM1 |
| Immunogen | A synthetic peptide derived from human VCAM1 (AA 700-739). |
| Positive Control | WB: NIH/3T3, HUVEC (treated or untreated with hTNFα) cell lysates. IHC: Human tonsil tissue. ICC/IF: K562 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human VCAM1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
VCAM1 Antibody (Ab133968) - Western Blot
All lanes: VCAM1 Antibody (Ab133968) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 103 kDa
Exposure time: 52.9 s
VCAM1 Antibody (Ab133968) - Western Blot
All lanes: VCAM1 Antibody (Ab133968) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 90 kDa
Exposure time: 15.9 s
VCAM1 Antibody (Ab133968) - IF/ICC
Immunofluorescence analysis of VCAM1 in K562 using VCAM1 Antibody (Ab133968), and cells were counterstained with DAPI (blue).
VCAM1 Antibody (Ab133968) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using VCAM1 Antibody (Ab133968). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| IF/ICC |
1/50-1/200
|
| WB | 1/1000 - 1/2000 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Feb 23, 2024 | Ab133968 | |
| Certificate of Analysis | Feb 23, 2024 | Ab133968 |