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Recombinant SDHB Antibody - Primary antibody, specific to SDHB, Rabbit IgG

COA COA
    Application:
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab126854
Grouped product items
SKU Size
Availability
 Price Qty
Ab126854-10μl
10μl
1
$59.90
Ab126854-50μl
50μl
1
$209.90
Ab126854-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
Ab126854-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human SDHB Antibody; WB, IP; Unconjugated

View related series
Click to view target information: SDHB
Accession#:P21912 (3) Gene ID:6390 (3) SDHB (3)

Basic Description

Product Name Recombinant SDHB Antibody - Primary antibody, specific to SDHB, Rabbit IgG
Synonyms CWS2 antibody | DHSB_HUMAN antibody | FLJ92337 antibody | Ip antibody | Iron sulfur subunit antibody | Iron sulfur subunit of complex II antibody | Iron-sulfur subunit of complex II antibody | mitochondrial antibody | PGL 4 antibody | PGL4 antibody | SDH
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Host species Rabbit
Specificity SDHB
Immunogen A synthetic peptide derived from human SDHB (AA 1-280).
Positive Control WB: Mouse slpeen, Rat spleen, HeLa, Jurkat and C6 lysates. IP: HeLa lysates.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SDHB Antibody, Recombinant, could be used for WB, IP and so on.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SDHB Tchem Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant SDHB Antibody (Ab126854) - Western Blot
All lanes: Recombinant SDHB Antibody (Ab126854) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 28, 24 kDa
The band observed at 24 kDa could potentially be a cleaved form of SDHB due to the presence of a 28 amino acid transit peptide.
Exposure time: 12.4 s

Recombinant SDHB Antibody (Ab126854) - IP
Immunoprecipitate (IP) analysis using Recombinant SDHB Antibody (Ab126854) at 1/50 dilution. (WB with Recombinant SDHB Antibody (Ab126854) at 1/1000 dilution).

Recombinant SDHB Antibody (Ab126854) - IF/ICC
IF analysis of Recombinant SDHB Antibody (Ab126854) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SDHB Antibody (Ab126854) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Application

Application Dilution info
WB

1/1000 - 1/2000
IP

1/20-1/50

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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