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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab121891-10μl
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10μl |
2
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$69.90
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Ab121891-50μl
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50μl |
1
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$199.90
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Ab121891-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
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Ab121891-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
|
Recombinant; Rabbit anti Human Pin1 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant Pin1 Antibody - Primary antibody, specific to PIN1, Rabbit IgG |
|---|---|
| Synonyms | EC 5.2.1.8 | Peptidyl-prolyl cis-trans isomerase Pin1 | PPIase Pin1 | Rotamase Pin1 | DOD antibody | DODO, Drosophila, homolog of antibody | FLJ40239 antibody | FLJ77628 antibody | MGC10717 antibody | NIMA interacting 1 antibody | Peptidyl prolyl cis tran |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizat |
| Host species | Rabbit |
| Specificity | PIN1 |
| Immunogen | Recombinant protein of human Pin1 (AA 1-165) |
| Positive Control | WB: Jurkat, HeLa, MCF7, Raji, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HEK293 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Pin1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant Pin1 Antibody (Ab121891) - Western Blot
All lanes: Recombinant Pin1 Antibody (Ab121891) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 14.3 s
Recombinant Pin1 Antibody (Ab121891) - IF/ICC
Immunocytochemistry analysis of PIN1 (green) in 293 using Recombinant Pin1 Antibody (Ab121891), and DAPI (blue).
Recombinant Pin1 Antibody (Ab121891) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Pin1 Antibody (Ab121891). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | May 27, 2024 | Ab121891 | |
| Certificate of Analysis | May 27, 2024 | Ab121891 |