Grouped product items

SKU

Size

Availability

Price

Qty

Ab120114-10μl

10μl

$59.90

Ab120114-50μl

50μl

$179.90

Ab120114-100μl

100μl

Available within 8-12 weeks(?)

$269.90

Ab120114-1ml

1ml

Available within 8-12 weeks(?)

$2,199.90

Basic Description

Product Name

Recombinant PAK2 Antibody - Primary antibody, specific to PAK2, Rabbit IgG

Synonyms

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.3 mg/mL

Host species

Rabbit

Specificity

PAK2

Immunogen

A synthetic peptide derived from human PAK2 (AA 1-13)

Positive Control

WB: K562, HeLa, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: HHuman tonsil tissue.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Product Description

Rabbit anti Human PAK2 Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500 - 1/1000

IHC: 1/50-1/100

Protein Function

The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.

Post-translational

Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Shape	Liquid
Concentration	0.3 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PAK2 Tchem Serine/threonine-protein kinase PAK 2 (0 Activities)

Discover Target: PAK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PAK2 Tchem Serine/threonine-protein kinase PAK 2 (1925 Activities)

Discover Target: PAK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PAK2 Tchem Serine/threonine-protein kinase PAK 1/PAK 2 (18 Activities)

Discover Target: PAK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant PAK2 Antibody (Ab120114)

Recombinant PAK2 Antibody (Ab120114) - Western Blot
All lanes: Recombinant PAK2 Antibody (Ab120114) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 59 kDa
Exposure time: 10.1 s

IHC: Recombinant PAK2 Antibody (Ab120114)

Recombinant PAK2 Antibody (Ab120114) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PAK2 Antibody (Ab120114). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application	Dilution info
WB	1/500-1/1000
IHC	1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0606456	Certificate of Analysis	Jun 04, 2024	Ab120114
ZJ24F0606455	Certificate of Analysis	Jun 04, 2024	Ab120114

Solution Calculators

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Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

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Recombinant PAK2 Antibody - Primary antibody, specific to PAK2, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

Recombinant PAK2 Antibody - Primary antibody, specific to PAK2, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews