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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab115571-10μl
|
10μl |
2
|
$66.90
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|
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Ab115571-50μl
|
50μl |
1
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$199.90
|
|
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Ab115571-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$299.90
|
|
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Ab115571-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
|
Recombinant; Rabbit anti Human MLKL Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant MLKL Antibody - Primary antibody, specific to MLKL, Rabbit IgG |
|---|---|
| Synonyms | hMLKL | Mixed lineage kinase domain like | Mixed lineage kinase domain like protein | Mixed lineage kinase domain-like protein | mixed lineage kinase domain like pseudokinase | MLKL_HUMAN | 9130019I15Rik antibody | FLJ34389 antibody | hMLKL antibody | Mix |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Host species | Rabbit |
| Specificity | MLKL |
| Immunogen | A synthetic peptide derived from human MLKL (AA 350-471) |
| Positive Control | WB: HUVEC, HT-29, HeLa, Jurkat, MCF7 and NIH/3T3 cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human MLKL Antibody, Recombinant, could be used for WB, IHC and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant MLKL Antibody (Ab115571) - Western Blot
All lanes: Recombinant MLKL Antibody (Ab115571) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 21.0 s
Recombinant MLKL Antibody (Ab115571) - IF/ICC
IF analysis of Recombinant MLKL Antibody (Ab115571) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MLKL Antibody (Ab115571) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| WB | 1/1000 - 1/2000 |
| IF/ICC |
1/200
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | May 14, 2024 | Ab115571 | |
| Certificate of Analysis | May 14, 2024 | Ab115571 |