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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab115427-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
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Ab115427-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
|
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Ab115427-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab115427-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,199.90
|
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Recombinant; Rabbit anti Human MiTF Antibody; WB, IF/ICC; Unconjugated
| Product Name | Recombinant MiTF Antibody - Primary antibody, specific to MITF, Rabbit IgG |
|---|---|
| Synonyms | bHLHe32 | Class E basic helix-loop-helix protein 32 | BHLHE32 antibody | Class E basic helix-loop-helix protein 32 antibody | CMM8 antibody | Homolog of mouse microphthalmiaantibody | Mi antibody | Microphthalmia associated transcription factor antibody | |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived |
| Host species | Rabbit |
| Specificity | MITF |
| Immunogen | A synthetic peptide derived from human MiTF (AA 350-526) |
| Positive Control | WB: HeLa, HEK293, A375, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human MiTF Antibody, Recombinant, could be used for WB, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant MiTF Antibody (Ab115427) - Western Blot
All lanes: Recombinant MiTF Antibody (Ab115427) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 50 - 70 kDa
The molecular weight observed is consistent with what has been described in the literature (PMID: 17182576).
Exposure time: 12.2 s
Recombinant MiTF Antibody (Ab115427) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MiTF Antibody (Ab115427) at 1/50 dilution.
Recombinant MiTF Antibody (Ab115427) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MiTF Antibody (Ab115427) at 1/50 dilution.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/50 - 1/200 |