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Recombinant MiTF Antibody - Primary antibody, specific to MITF, Rabbit IgG

    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab115427
Grouped product items
SKU Size
Availability
Price Qty
Ab115427-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab115427-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab115427-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab115427-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

Recombinant; Rabbit anti Human MiTF Antibody; WB, IF/ICC; Unconjugated

Basic Description

Product Name Recombinant MiTF Antibody - Primary antibody, specific to MITF, Rabbit IgG
Synonyms bHLHe32 | Class E basic helix-loop-helix protein 32 | BHLHE32 antibody | Class E basic helix-loop-helix protein 32 antibody | CMM8 antibody | Homolog of mouse microphthalmiaantibody | Mi antibody | Microphthalmia associated transcription factor antibody |
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived
Host species Rabbit
Specificity MITF
Immunogen A synthetic peptide derived from human MiTF (AA 350-526)
Positive Control WB: HeLa, HEK293, A375, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human MiTF Antibody, Recombinant, could be used for WB, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MITF Tchem Microphthalmia-associated transcription factor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant MiTF Antibody (Ab115427) - Western Blot
All lanes: Recombinant MiTF Antibody (Ab115427) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 50 - 70 kDa
The molecular weight observed is consistent with what has been described in the literature (PMID: 17182576).
Exposure time: 12.2 s

Recombinant MiTF Antibody (Ab115427) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MiTF Antibody (Ab115427) at 1/50 dilution.

Recombinant MiTF Antibody (Ab115427) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MiTF Antibody (Ab115427) at 1/50 dilution.

Application

Application Dilution info
WB

1/1000 - 1/2000

IF/ICC

1/50 - 1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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