Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab104881-10μl
|
10μl |
5
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$59.90
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Ab104881-50μl
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50μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$177.90
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Ab104881-100μl
|
100μl |
1
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$299.90
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Ab104881-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$2,307.90
|
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Recombinant; Rabbit anti Human GATA3 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant GATA3 Antibody - Primary antibody, for WB,IHC various applications, from Human, generated in Rabbit, specific to GATA3, Rabbit IgG |
|---|---|
| Synonyms | GATA 3 antibody | GATA binding factor 3 antibody | GATA binding protein 3 antibody | GATA-binding factor 3 antibody | Gata3 antibody | GATA3_HUMAN antibody | HDR antibody | HDRS antibody | MGC2346 antibody | MGC5199 antibody | MGC5445 antibody | Trans act |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.2mg/mL |
| Host Species | Rabbit |
| Host species | Rabbit |
| Specificity | GATA3 |
| Application | WB,IHC |
| Immunogen | A synthetic peptide derived from human GATA3 (AA 1-220). |
| Positive Control | WB: Jurkat, HEK293 and K562 cell lysates. IHC: Human bladder cancer tissues. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human GATA3 Antibody, Recombinant, could be used for WB and IHC. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Rabbit IgG |
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | Cell supernatant |
| Shape | Liquid |
| Concentration | 1.2mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant GATA3 Antibody (Ab104881) - Western Blot
All lanes: Recombinant GATA3 Antibody (Ab104881) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48&37 kDa
Specific bands were detected for GATA3 full length at approximately 48kDa and the splice form at approximately 37kDa.
Exposure time: 2.3 s
Recombinant GATA3 Antibody (Ab104881) - Western Blot
All lanes: Recombinant GATA3 Antibody (Ab104881) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48&37 kDa
Specific bands were detected for GATA3 full length at approximately 48kDa and the splice form at approximately 37kDa.
Exposure time: 4.9 s
Recombinant GATA3 Antibody (Ab104881) - IHC
Immunohistochemistry analysis of paraffin-embedded Human bladder cancer using Recombinant GATA3 Antibody (Ab104881)(1/50). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 14, 2023 | Ab104881 | |
| Certificate of Analysis | Aug 14, 2023 | Ab104881 |