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Recombinant DNA Ligase IV/LIG4 Antibody - Primary antibody, specific to LIG4, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab100073
Grouped product items
SKU Size
Availability
 Price Qty
Ab100073-10μl
10μl
1
$69.90
Ab100073-50μl
50μl
1
$209.90
Ab100073-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab100073-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human DNA Ligase IV/LIG4 Antibody; WB, ICC, IF; Unconjugated

View related series
Click to view target information: LIG4
Accession#: P49917 (1) DNA Ligase IV (1) Gene ID: 3981 (1) LIG4 (1)

Basic Description

Product Name Recombinant DNA Ligase IV/LIG4 Antibody - Primary antibody, specific to LIG4, Rabbit IgG
Synonyms DNA joinase antibody | DNA ligase 4 antibody | DNA ligase IV antibody | DNA repair enzyme antibody | DNLI4_HUMAN antibody | LIG 4 antibody | LIG4 antibody | LIG4S antibody | DNA ligase IV ATP dependent antibody | Ligase IV antibody | Ligase IV DNA ATP dep
Specifications & Purity Recombinant, ExactAb™, KD Validation, Validated, See COA
Host species Rabbit
Specificity LIG4
Immunogen A synthetic peptide derived from human DNA Ligase IV (AA 504-685).
Positive Control WB: Hep G2, Ramos and MCF7 cell lysates. IF/ICC: HeLa cells
Conjugation Unconjugated
Grade ExactAb™, KD Validation, Recombinant, Validated
Product Description

Rabbit anti Human DNA Ligase IV/LIG4 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
Protein Function:
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

LIG4 Tbio DNA ligase 4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
LIG4 Tbio LIG4/XRCC4 (1 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - Western Blot
All lanes: Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 103 kDa
Observed band size: 98 kDa
Exposure time: 60.0 s

Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - Western Blot
All lanes: Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 103 kDa
Observed band size: 102 kDa
Exposure time: 9.2 s

Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - Western Blot
All lanes use Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/1000 dilution for 1 hour at room temperature.

Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) - IF/ICC
Immunofluorescent analysis of Hela cells, using Recombinant DNA Ligase IV/LIG4 Antibody (Ab100073) at 1/50 dilution.

Application

Application Dilution info
IF/ICC

1/50-1/200

WB

1/500-1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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